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Int J Clin Exp Pathol 2010;3(5):710-717

Original Article
Specific inhibition of HepG2 cells proliferation through apoptosis induced by gabexate
mesilate

Tsuneo Ozeki, Tsuneo Natori  

Ozeki Internal Medicine Clinic, 10-30-10, Iseigaoka, Nishiku Yahata, Kitakyushu City, Japan; SRL, Inc, Hamura Laboratory, 3-5-5 Midorigaoka
Hamura City, Tokyo, Japan.

Received April 12, 2010; accepted August 12, 2010; available online August 15, 2010

Abstract: Many anticancer drugs are developing until now. However, conventional anticancer drugs causes damage to not only cancer cells but
also non-cancerous tissues and cells. Therefore, the development of new drugs are anticipated.HepG2 cell proliferation in cell culture was
significantly inhibited by gabexate mesilate. In TUNEL method, a significant amount of HepG2 cells cultured with gabexate mesilate showed a
decrease in the number of total cells and an increased in the number of positive cells. Further immunohistochemical staining for P-53,ss-DNA
and caspase 3 showed samely a decrease in the number of total cells and an increase in the number of positive cells. The staining for bcl2
showed a decrease in the number of total cells and no remarkable change in the number of positive cells. The cell growth inhibition by
gabexate mesilate was almost blocked by caspase 3 inhibitor. Therefore, the inhibition itself of HepG2 cell proliferation by gabexate mesilate
was mainly due to the apoptosis. This agent causes mainly damage to HepG2 cell by apoptosis but does not cause side effects, differing from
the above anticancer drugs, Gabexate mesilate is a useful drug. (IJCEP1004003).

Key words: Apoptosis,hepatocellular carcinoma, HepG2cell, gabexate mesilate

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Tsuneo Ozeki, MD
Ozeki Internal Medicine Clinic
10-30-10, Iseigaoka, Nishiku Yahata
Kitakyushu City, Japan
Tel: 093 693 0712, Fax: 093 693 0712
E-mail:
tsuneo_ozeki@yahoo.co.jp