Original Article Skeletal muscle-derived stem cells differentiate into hepatocyte-like cells and aid in liver regeneration
Ian H Bellayr, Burhan Gharaibeh, Johnny Huard, Yong Li,
Stem Cell Research Center, Orthopaedic Surgery Department & Children’s Hospital of UPMC; Department of Bioengineering, University of Pittsburgh; Department of Orthopaedic Surgery, University of Pittsburgh, School of Medicine; Department of Pathology, University of Pittsburgh, School of Medicine, Pittsburgh, PA 15219, USA.
Received July 14, 2010, accepted July, available online July, 2010
Abstract: The liver is unique for its ability to regenerate after injury, however, critical injuries or disease cause it to lose this quality. Stem cells have been explored as a possibility to restore the function of seriously damaged livers, based on their self-renewability and multiple differentiation capacity. These experiments examine the ability of muscle derived stem cells (MDSCs) to differentiate into hepatocyte-like cells in vitro and acquire functional liver attributes for repairing damaged livers. In vitro experiments were performed using MDSCs from postnatal mice and mouse hepatocyte cell lines. Our data revealed that MDSCs differentiated into hepatocyte-like cells and expressed liver cell markers, albumin, hepatocyte nuclear factor 4α, and alpha feto-protein, both at the RNA and protein level. Additionally, in vivo studies showed successful engraftment of MDSCs into hepatectomized mouse livers of mice. These results provide evidence suggesting that MDSCs have the capacity to differentiate into liver cell-like cells and may serve as potential candidates to aid in liver regeneration. (IJCEP1007005).
Address all correspondence to: Yong Li, MD, PhD Department of Orthopaedic Surgery, Pathology and Bioengineering University of Pittsburgh Director, Laboratory of Molecular Pathology Suite 206 Bridgeside Point II 450 Technology Drive Pittsburgh, PA 15219 Tel: 412-648-3313 Fax: 412-648-4066 E-mail: firstname.lastname@example.org