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Int J Clin Exp Pathol 2011;4(1):74-84

Original Article
Effect of exogenous galectin-1 on leukocyte migration: modulation of cytokine levels and
adhesion molecules

Cristiane D. Gil, Caio E. Gullo, Sonia M. Oliani

Department of Anatomy, São José do Rio Preto School of Medicine – FAMERP, São José do Rio Preto, SP, Brazil; Department of Morphology
and Genetics, Federal University of São Paulo – UNIFESP, São Paulo, SP, Brazil; Department of Biology, Instituto de Biociências, Letras e
Ciências Exatas, São Paulo State University - UNESP, São José do Rio Preto, SP, Brazil.

Received December 7, 2010; accepted December 18, 2010; Epub December 20, 2010; published January 1, 2011

Abstract: The effect of exogenous Gal-1 on cellular response and adhesion molecule expression was investigated in a classical model of
acute inflammation induced by zymosan. C57BL6 mice, treated or not with human recombinant (hr) Gal-1, received i.p. injection of zymosan and
peritoneal exudate, blood and mesentery were processed for cellular, biochemical, light and electron microscopic analysis after 4 and 24 h.
Zymosan peritonitis provoked the expected signs of inflammation at 4 h, including a significant increase in extravasated PMNs in the
mesentery and peritoneal exudate, mirrored by blood neutrophilia. These changes subsided after 24 h. Ultrastructural immunocytochemical
analysis of PMNs showed significant Gal-1 expression and co-localization with L-selectin and β2-integrin in the plasma membrane and
cytoplasm. Pharmacological treatment with hrGal-1 at 4 h produced an inhibition of PMN migration, associated with diminished expression of
adhesion molecules, particularly β2- integrin, and TNF-α and IL-1β release by peritoneal cells. At 24 h, Gal-1 induced an increase in
mononuclear phagocytic cell recruitment. In conclusion, our data propose an important mechanism of anti-inflammatory action of Gal-1, initially
by modulation of pro-inflammatory cytokine release and PMN migration through an imbalance between adhesion molecule expression and,
later, by promoting monocyte-macrophage recruitment. (IJCEP1012003).

Keywords: CD11b, CD62L, monocyte, neutrophil, zymosan peritonitis, immunocytochemistry.

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Address all correspondence to:
Dr. Sonia M. Oliani
Department of Biology, Instituto de Biociências
Letras e Ciências Exatas, São Paulo State University (UNESP)
Rua Cristóvão Colombo 2265, 15054-000
São José do Rio Preto, SP, Brazil.
Tel: +55-17-32212381, Fax: +55-17-32212390
E-mail:
smoliani@ibilce.unesp.br


Dr. Cristiane D. Gil
Department of Morphology and Genetics
Federal University of São Paulo – UNIFESP,
Rua Botucatu 740, 04023-900, São Paulo
SP, Brazil.
Tel/Fax: +55-11-55764268
E-mail:
cristiane.gil@unifesp.br