Original Article Effect of exogenous galectin-1 on leukocyte migration: modulation of cytokine levels and adhesion molecules
Cristiane D. Gil, Caio E. Gullo, Sonia M. Oliani
Department of Anatomy, São José do Rio Preto School of Medicine – FAMERP, São José do Rio Preto, SP, Brazil; Department of Morphology and Genetics, Federal University of São Paulo – UNIFESP, São Paulo, SP, Brazil; Department of Biology, Instituto de Biociências, Letras e Ciências Exatas, São Paulo State University - UNESP, São José do Rio Preto, SP, Brazil.
Received December 7, 2010; accepted December 18, 2010; Epub December 20, 2010; published January 1, 2011
Abstract: The effect of exogenous Gal-1 on cellular response and adhesion molecule expression was investigated in a classical model of acute inflammation induced by zymosan. C57BL6 mice, treated or not with human recombinant (hr) Gal-1, received i.p. injection of zymosan and peritoneal exudate, blood and mesentery were processed for cellular, biochemical, light and electron microscopic analysis after 4 and 24 h. Zymosan peritonitis provoked the expected signs of inflammation at 4 h, including a significant increase in extravasated PMNs in the mesentery and peritoneal exudate, mirrored by blood neutrophilia. These changes subsided after 24 h. Ultrastructural immunocytochemical analysis of PMNs showed significant Gal-1 expression and co-localization with L-selectin and β2-integrin in the plasma membrane and cytoplasm. Pharmacological treatment with hrGal-1 at 4 h produced an inhibition of PMN migration, associated with diminished expression of adhesion molecules, particularly β2- integrin, and TNF-α and IL-1β release by peritoneal cells. At 24 h, Gal-1 induced an increase in mononuclear phagocytic cell recruitment. In conclusion, our data propose an important mechanism of anti-inflammatory action of Gal-1, initially by modulation of pro-inflammatory cytokine release and PMN migration through an imbalance between adhesion molecule expression and, later, by promoting monocyte-macrophage recruitment. (IJCEP1012003).
Address all correspondence to: Dr. Sonia M. Oliani Department of Biology, Instituto de Biociências Letras e Ciências Exatas, São Paulo State University (UNESP) Rua Cristóvão Colombo 2265, 15054-000 São José do Rio Preto, SP, Brazil. Tel: +55-17-32212381, Fax: +55-17-32212390 E-mail: email@example.com
Dr. Cristiane D. Gil Department of Morphology and Genetics Federal University of São Paulo – UNIFESP, Rua Botucatu 740, 04023-900, São Paulo SP, Brazil. Tel/Fax: +55-11-55764268 E-mail: firstname.lastname@example.org