Original Article D2-40: an additional marker for myoepithelial cells of breast and the precaution in interpreting tumor lymphovascular invasion
Shuyue Ren, Mohammed Abuel-Haija, Jasvir S. Khurana, Xinmin Zhang
Department of Pathology and Laboratory Medicine, Temple University Hospital, Temple University School of Medicine, Philadelphia, PA 19140, USA.
Received January 12, 2011; accepted January 29, 2011; Epub January 30, 2011; published February 15, 2011
Abstract: D2-40 is a recently available mouse monoclonal antibody specifically recognized human podoplanin and has been used in identifying lymphovascular invasion (LVI) of tumors. Although its expression has been evaluated in other tissues, as a marker of the myoepithelial cells (MEC) of breast it has not been studied. To explore its expression in the MEC of breast, histological materials of 48 patients with breast diseases were reviewed and paraffin embedded tissue blocks were selected to include usual ductal hyperplasia ( UDH, 41 cases), atypical ductal hyperplasia (ADH, 4 cases) and ductal carcinoma in situ (DCIS, 17 cases). Normal breast parenchyma and invasive carcinoma coexisting in the tissue sections were also included in the study. Immunohistochemistry for D2-40, calponin and p63 was performed and the staining patterns were reviewed and compared. D2-40 immunohistochemical staining is positive in the cytoplasm of MEC at UDH, ADH, and majority of DCIS. The staining pattern of D2-40 is compatible with that of calponin, however D2-40 staining of MEC is weaker than that of calponin and with less background. In addition, myoepithelial cells and myofibroblasts at the edge of retraction space of DCIS are also stained by D2-40 that could be misinterpreted as tumor LVI. In conclusion, D2-40 immunohistochemistry reliably identifies the MEC of breast in a variety of lesions in a pattern similar to that of calponin and p63, and can be used as an additional MEC marker. Precaution should be excised when interpreting the staining of cells surrounding DCIS and carcinoma with retraction artifact. (IJCEP1101001).
Address all correspondence to: Xinmin Zhang, MD Department of Pathology and Laboratory Medicine 3401 North Broad Street, Rm 350/OPB Philadelphia, PA 19140, USA. Tel: 215 -707-8273 E-mail: firstname.lastname@example.org