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Int J Clin Exp Pathol 2011;4(3):276-286

Original Article
CXCL12 controls over-invasion of trophoblasts via up-regulating CD82 expression in
DSCs at maternal-fetal interface of human early pregnancy in a paracrine manner

Ming-Qing Li, Chuan-Lin Tang, Mei-Rong Du, Deng-Xuan Fan, Hong-Bo Zhao, Bing Xu, Da-Jin Li

Laboratory for Reproductive Immunology, Hospital and Institute of Obstetrics and Gynecology, Fudan University Shanghai Medical College,
Shanghai, 200011,China; Department of Obstetrics and Gynecology, The Affiliated Hospital, Hainan Medical College, Haikou, 571101, China.

Received March 3, 2011; accepted March 19, 2011; Epub March 20, 2011; published March 31, 2011

Abstract: Tetraspanin CD82 has been identified as a potential contributor to controlling trophoblast invasiveness in human first-trimester
pregnancy. However, it is unclear how the regulation of CD82 expression at maternal-fetal interface. The present study is to investigate the
effect of the trophoblast-derived CXCL12 on CD82 expression in decidual stromal cells (DSCs) that in turn controls trophoblast cell
invasiveness. In-cell Western was used to evaluate the expression of CD82 in DSCs. A co-culture model was established to investigate the
reciprocal interaction between trophoblasts and DSCs via CXCL12/CXCR4 and CD82 expression. We found that both anti-CXCL12 and
anti-CXCR4 neutralizing antibody can eliminate increase of CD82 expression in DSCs induced by the trophoblasts supernatant. Moreover, the
invasiveness of trophoblasts pre-treated with anti-CXCR4 neutralizing antibody was significantly decreased. Interestingly, when DSCs were
pre-treated with anti-CXCR4 neutralizing antibody, the trophoblasts invasiveness in the co-culture was enhanced, and thus anti-CXCR4
neutralizing antibody can reverse the decrease of trophoblasts invasiveness induced by CD82. The trophoblast cell-derived CXCL12 does not
only increase the invasiveness in an autocrine manner, but also control the over-invasion of trophoblasts through promoting CD82 expression
in DSCs in a paracrine manner, which maintains a physiological balance of human trophoblasts invasiveness via the cross-talk between
trophoblasts and DSCs. (IJCEP1103002).

Keywords: CXCL12, CD82, trophoblasts, DSCs, invasiveness

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Address all correspondence to:
Dr. Da-jin Li
Laboratory for Reproductive Immunology
Hospital and Institute of Obstetrics and Gynecology
Fudan University Shanghai Medical College
Shanghai, 200011,China
Tel: 86-21-63457331; Fax: 86-21-63457331
E-mail:
djli@shmu.edu.cn