Department of Neurology, The fourth hospital of Harbin medical university, Heilongjiang , 150001,China; Department of Neurology,The first hospital of Jilin university, Jilin,130021,China; Department of anesthesiology, The first hospital of Jilin university, Jilin ,130021,China; Peking university Shenzhen hospital, Shenzhen, 518036, China.
Received May 10, 2011; accepted May, 2011; Epub May, 2011, published May, 2011
Abstract: Stroke is one of the neurological diseases which lead to permanently neuronal damage after temporary or long-term occlusion of vessels or after heart attack. However, there are few efficient strategies to prevent or treat this kind of insult in clinical because once the onset of stroke, the consequence is irreversible and could be long-lasting. Gene therapy especially using viral system has long been addressed to be of great potential to reduce the damage. Here, we generated recombinant adeno-associated virus (rAAV) carrying brain-derived neurotrophic factor (BDNF) gene. Cells infected with rAAV-BDNF could be able to produce functional BDNF which promoted neurite outgrowth and protected neurons from apoptosis induced by serum deprivation. Further more, single injection of rAAV has neuroprotection against cell death in focal ischemic model. These results showed that rAAV-mediated gene delivery is functional and of relevance, which shed light to the future application of viral system-based gene therapy in clinical. (IJCEP1105003)
Address all correspondence to: Jiexu Zhao, MD, PhD Department of Neurology The first hospital of Jilin university Jilin,130021, Peoples Republic of China Tel. +86 (28) 85532494 Fax. +86 (28) 85559065 E-mail: email@example.com