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Int J Clin Exp Pathol 2011;4(5):496-504

Original Ariticle
rAAV-mediated delivery of brain-derived neurotrophic factor promotes neurite outgrowth
and protects neurodegeneration in focal ischemic model

Jingyu Zhang, Zhigang Yu, Zhiqiang Yu, Zichao Yang, Hong Zhao, Luran Liu, Jiexu Zhao

Department of Neurology, The fourth hospital of Harbin medical university, Heilongjiang , 150001,China; Department of Neurology,The first
hospital of Jilin university, Jilin,130021,China; Department of anesthesiology, The first hospital of Jilin university, Jilin ,130021,China; Peking
university Shenzhen hospital, Shenzhen, 518036, China.

Received May 10, 2011; accepted May, 2011; Epub May, 2011, published May, 2011

Abstract: Stroke is one of the neurological diseases which lead to permanently neuronal damage after temporary or long-term occlusion of
vessels or after heart attack. However, there are few efficient strategies to prevent or treat this kind of insult in clinical because once the onset of
stroke, the consequence is irreversible and could be long-lasting. Gene therapy especially using viral system has long been addressed to be
of great potential to reduce the damage. Here, we generated recombinant adeno-associated virus (rAAV) carrying brain-derived neurotrophic
factor (BDNF) gene. Cells infected with rAAV-BDNF could be able to produce functional BDNF which promoted neurite outgrowth and protected
neurons from apoptosis induced by serum deprivation. Further more, single injection of rAAV has neuroprotection against cell death in focal
ischemic model. These results showed that rAAV-mediated gene delivery is functional and of relevance, which shed light to the future
application of viral system-based gene therapy in clinical. (IJCEP1105003)

Keywords: Stroke, recombinant adeno-associated virus, brain-derived neurotrophic factor, gene therapy

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Address all correspondence to:
Jiexu Zhao, MD, PhD
Department of Neurology
The first hospital of Jilin university
Jilin,130021, Peoples Republic of China
Tel. +86 (28) 85532494
Fax. +86 (28) 85559065