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Int J Clin Exp Pathol 2012;5(2):137-39

Brief Communication
Pitfalls in immunohistochemistry - a recent example

Alexander Riechers, Anja Katrin Bosserhoff

Institute of Pathology, University of Regensburg, Germany

Received January 31, 2012; accepted February 7, 2012; Epub February 12, 2012; Published February 28, 2012

Abstract: Immunohistochemistry is an important and valuable technique in many fields of research, although several common pitfalls can lead
to wrong or misinterpreted results. A recently published study [1] claims that the protein MIA (melanoma inhibitory activity) is expressed in
Purkinje cells in the cerebellum. Careful re-analysis resulted in negative results. Due to these results of our group we feel that this analysis
could serve as example for the potential problems in immunohistochemistry caused by the combination of an unspecific antibody and the
omission of evaluating control tissue samples.

Keywords: Immunohistochemistry, antibody, CD-RAP, MIA, specificity

Address all correspondence to:
Dr. Anja Katrin Bosserhoff
Institute of Pathology, University of Regensburg
Franz-Josef-Strauss-Allee 11, D-93053
Regensburg, German.
Tel: (+49)-941-944-6705; Fax: (+49)-941-944-6602
E-mail: anja.bosserhoff@klinik.uni-regensburg.de