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Int J Clin Exp Pathol 2012;5(3):216-223
Morphoproteomic confirmation of an activated nuclear factor-кBp65 pathway in follicular
Jing Liu, Robert E. Brown
Department of Pathology and Laboratory Medicine, University of Texas Health Science Center at Houston Medical School, Houston, Texas
Received February 10, 2012; accepted March 3, 2012; Epub March 25, 2012; Published March 30, 2012
Abstract: The role of nuclear factor (NF)-кBp65 pathway in the pathogenesis of follicular thyroid carcinoma (FTC) has not been fully
investigated. We retrieved 10 cases of FTC from our file. Tissue microarrays (TMAs) were constructed using 2.0 mm cores from formalin-fixed,
paraffin-embedded tissue blocks. TMA sections were immunohistochemically stained for phosphorylated (p)-NF-кBp65 (Ser 536),
cyclooxygenase-2 (COX-2), IL-8, and glutathione S-transferase (GST)-pi. Staining intensity (0-3+), extensiveness (0-100%) and subcellular
compartmentalization were evaluated. Both nuclear and cytoplasmic immunoreactivities with p-NF-кBp65 (Ser 536) antibodies were observed
in all 10 cases, including moderate to strong nuclear staining intensity with a range of extensiveness in 20% - 100% of tumor cells. Moderate
(2+) or strong (3+) cytoplasmic expressions of COX-2 and IL-8 were present in 60-100% and 50-100% of tumor cells, respectively, in all cases.
GST-pi was diffusely (70-100%) and moderately or strongly staining the tumor cytoplasm in all cases (except one case with insufficient tissue)
with three of them demonstrating nuclear positivity as well. Morphoproteomic analysis reveals the constitutive activation of the NF-кBp65
pathway in follicular thyroid carcinomas as evidenced by phosphorylation at Ser 536 with nuclear translocation and with correlative expression
of transcriptionally activated gene products (COX-2, IL-8, and GST-pi). This observation may provide a molecular basis for the tumor biology
and targeted therapies for follicular thyroid carcinoma. (IJCEP1202004).
Keywords: Anticardiolipin, APhL, antiphospholipid antibodies, method comparison
Address all correspondence to:
Dr. Jing Liu
Department of Pathology and Laboratory Medicine
University of Texas Health Science Center at Houston Medical School
6431 Fannin Street, MSB 2.260A, Houston, Texas, USA.
Tel: (713) 500-5327; Fax: (713) 500-0695