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Int J Clin Exp Pathol 2012;5(3):224-230
Utility of peripheral blood flow cytometry in differentiating low grade versus high grade
myelodysplastic syndromes (MDS) and in the evaluation of cytopenias
Hooman H Rashidi, Xiangdong Xu, Huan-You Wang, Nelofar Q Shafi2 Karuna Rameshkumar, Karen Messer, Brian R Smith, Michal G Rose
University of California San Diego School of Medicine & VA Medical Center San Diego, USA; Yale University School of Medicine and Connecticut
VA Medical Center, USA; Department of Clinical Pathology, St. John’s Medical College Hospital, Bangalore, India; Division of Biostatistics,
Moores UCSD Cancer Center, CA, USA
Received February 28, 2012; accepted March 16, 2012; Epub March 25, 2012; Published March 30, 2012
Abstract: The diagnostic utility of flow cytometry in the evaluation of cytopenias and in the differential diagnosis of low-grade versus high-grade
myelodysplastic syndrome (MDS) is not widely appreciated. In this report, we measured granulocyte CD10/control fluorescence ratio in 29
patients with MDS & chronic myelomonocytic leukemia (CMML) using peripheral blood (PB) flow cytometry (FC). We found a lower ratio in high-
grade MDS and CMML (mean ratio of 2.2 ± 0.7) vs. low-grade MDS (3.65 ± 0.9) and 16 cytopenic controls without MDS (3.67 ± 0.65; p<0.001).
The sensitivity and specificity of CD10 ratio <3 for the group that included the high risk MDS and CMML patients were 87.5% and 100%,
respectively. Our data suggests that FC of PB may be helpful in the work-up of patients with cytopenias and in the differential diagnosis of low-
grade vs. high-grade MDS. (IJCEP1202012).
Keywords: Myelodysplastic syndromes, flow cytometry, CD10, high-grade MDS, low-grade MDS, bone marrow, peripheral blood
Address all correspondence to:
Dr. Hooman H Rashidi
University of California San Diego School of Medicine & VA Medical Center
San Diego, USA;
Dr. Michal G Rose
Yale University School of Medicine and Connecticut VA Medical Center,