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Int J Clin Exp Pathol 2012;5(4):290-298

Original Article
Lentivirus-mediated RNAi knockdown of VEGFA in RKO colorectal cancer cells
decreases tumor formation and growth in vitro and in vivo

Jiang-Feng Qiu, Zhi-Qi Zhang, Yong Wang, Jun You

Department of General Surgery, Ningbo First Hospital, Ningbo, 315010, China; Department of Surgical Oncology, the First affiliated Hospital of
Xiamen University, Xiamen, 361003, China. *Jiang-Feng Qiu and Zhi-Qi Zhang contributed equally to this work.

Received March 27, 2012; accepted April 23, 2011; Epub April 30, 2011; published May 30, 2012

Abstract: Vascular endothelial growth factors (VEGF) play important roles in angiogenesis, vasculogenesis and endothelial cell growth. In
endothelial cancers, secreted VEGF proteins induce endothelial cell proliferation, promote cell migration, inhibit apoptosis and induce blood
vessel permeabilization. VEGFA is frequently overexpressed in human colorectal cancers (CRC) and its expression correlates with tumor
progression and invasiveness. In this study we examine the effect of knocking down VEGFA expression by infecting RKO colorectal cancer cells
with lentiviral particles containing VEGFA-targeting RNAi constructs. We found that suppressing VEGFA dramatically decreased RKO cell
proliferation, colony formation, invasion, migration and tumor growth. Furthermore, VEGFA knock-down reduced MAPK pathway signaling and
Smac/DIABLO expression. These results suggest that lentivirus-mediated RNAi knock-down of VEGFA could be an effective therapy for the
treatment of CRC.

Keywords: vascular endothelial growth factor A, shRNA, knock-down, colorectal cancer, lentivirus

Address all correspondence to:
Jiang-Feng Qiu, PhD
Department of General Surgery
Ningbo First Hospital
Ningbo, 315010, P.R. China
Tel: 0086-0574-87089859
Fax: 0086-0574-87304070
E-mail: jfqiu2011@gmail.com