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Int J Clin Exp Pathol 2012;5(6):537-546

Original Article
Analysis of whole genomic expression profiles and screening of the key signaling
pathways associated with pancreatic cancer

Chengzhi He, Hua Jiang, Shasha Geng, Haihui Sheng, Xiaoying Shen, Xiaoyan Zhang, Shizhang Zhu, Ximei Chen, Changqing Yang, HengJun

Department of Gastroenterology, Shanghai East Hospital Affiliated to Tongji University, Shanghai 200120, China; 2Department of
Gastroenterology, Institute of Digestive Diseases, Tongji Hospital Affiliated to Tongji University, Shanghai 200065, China; 3National
Engineering Center for Biochip at Shanghai, 201203, China

Received May 24, 2012; Accepted July 1, 2012; Epub July 29, 2012; Published August 15, 2012

Abstract: The tumorigenesis of pancreatic cancer is thought to be a complex process. Investigation of the molecular mechanism of pancreatic
cancer and exploring the specific markers for early diagnosis and specific targets of therapy is a key point to prevent and treat pancreatic cancer
effectively and to improve their prognosis. In this study, expression profiles experiment was performed using Agilent human whole genomic
oligonucleotide microarrays with 41,000 genes. Differentially expressed genes related with pancreatic cancer were screened, and analyzed
further by GO term analysis and KEGG Pathway analysis. Our results showed that there were 1276 differentially expressed genes associated
with pancreatic cancer. 691 genes were up regulated and 585 were down regulated in pancreatic cancer group. The present study confirmed
that the occurrence of pancreatic cancer was involved in multiple-gene interaction. In addition, our study found that pancreatic cancer was
related to an activation of the mTOR signaling pathway and renal cell carcinoma pathway. (IJCEP1205010).

Keywords: Pancreatic cancer, microarray, gene expression profile, signaling pathway

Address all correspondence to:
Dr. Hua Jiang
Department of Gastroenterology
Shanghai East Hospital Affiliated to Tongji University
Shanghai 200120, China.
E-mail: huajiang666@yahoo.com.

Dr. Hengjun Gao
Department of Gastroenterology
Institute of Digestive Diseases
Tongji Hospital Affiliated to Tongji University
Shanghai 200065, China.
National Engineering Center for Biochip
Shanghai, 201203, China.
Tel: 86-021-51320287; Fax: 86-021-51320287
E-mail: hengjun_gao@shbiochip.com