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Int J Clin Exp Pathol 2012;5(6):555-561

Original Article
Knockdown of apoptosis repressor with caspase recruitment domain (ARC) increases
the sensitivity of human glioma cell line U251MG to VM-26

Qiong Wang*, Ailin Li*, Hong Wang, Jinhuan Wang

Tian Jin Medical University , Tianjin 300070, China; Tianjin Institute of Neurosurgery, Huanhu Hospital of Tianjin, Tianjin 300060, China. *Both
authors contribute equally to this work.

Received May 27, 2012; Accepted June 28, 2012; Epub July 29, 2012; Published August 15, 2012

Abstract: Previous studies have demonstrated that apoptosis repressor with caspase recruitment domain (ARC) is up-regulated in many
forms of malignant tumors and low levels of ARC protein were expressed in normal human brain tissue. Little is known expression of ARC in
glioma. Here, we found that ARC protein is highly expressed in primary human glioma when compared with normal brain tissues. A decrease
in cell viability and an increase in apoptosis were observed in U251MG cells after ARC was knocked down. Knockdown of ARC was confirmed
by western blotting. Knockdown of ARC promoted caspase-8, caspase-3 activation and Bax accumulation. These results indicated that ARC
has a anti-apoptosis function in glioma. (IJCEP1205012)

Keywords: ARC, glioma, Apoptosis, VM-26 sensitivity

Address all correspondence to:
Dr. Jinhuan Wang
Tianjin Institute of Neurosurgery
Huanhu Hospital of Tianjin
122 Qixiangtai street
Tianjin 300060, Tianjin, China.
Tel: +86-22-60367668; Fax: 86-22-60367651
E-mail: wangjinhuanfch@yahoo.com.cn