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Int J Clin Exp Pathol 2012;5(9):965-971
Clinicopathological significance of PTEN and bcl2 expressions in oral squamous cell
Arshad Rrahmani, Mohammad Alzohairy, Ali Yousif Babiker, Moshahid A Rizvi, Husain Gad Elkarimahmad
Department of Medical laboratory science, College of Applied Medical Sciences, Qassim University, Kingdom of Saudi Arabia; Department of
Bioscienes, Jamia Millia Islamia, New Delhi, India; Department of Histopathology and Cytology, College of Medical Laboratories Science,
University of Khartoum, Sudan.
Received July 7, 2012; Accepted September 17, 2012; Epub October 20, 2012; Published October 30, 2012
Abstract: A high frequency of mutations at the PTEN locus has been noticed in carcinoma of oral. However, the role of PTEN alternations and
its association with outcome variables in the genesis of oral carcinoma is not understood fully. The purpose of our study was to examine the
impact of PTEN and Bcl2 in the genesis of Squamous cell carcinoma of oral. Total numbers of 60 histopathologically confirmed cases of
Squamous Cell Carcinoma and 15 cases of inflammatory lesion of oral specimens were studied. We assessed PTEN and bcl2
overexpression by the use of anti-PTEN and anti-bcl2 antibody through immunohistochemistry as directed by the manufacturer. There was
progressive loss of PTEN expression from inflammatory lesion to OSCC (p<0.05). Significant differences were found for PTEN expression
between inflammatory lesion and OSCC. The difference in expression pattern of PTEN in gender did not reach statistical significance (p>0.05).
The expression of bcl2 was found to be restricted to tumor cells in well and moderately differentiated tumors. The intense expression of bcl2
was observed throughout the tumor cell in poorly differentiated tumors.The Overexpression of bcl2 and loss of PTEN expression were
correlated to poor differentiation, lymph node involvement and late stages. Thus, alteration of PTEN and bcl2 is likely an important molecular
event in pathogenesis and carcinogenesis of oral carcinoma. (IJCEP1207005).
Keywords: Bcl-2, apoptosis, squamous cell carcinoma, IHC
Address all correspondence to:
Dr. Arshad Rahmani
Department of Medical Laboratory science
College of Applied Medical Sciences
Kingdom of Saudi Arabia.