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Int J Clin Exp Pathol 2013;6(3):503-509

Original Article
Effect of glutamine on apoptosis of intestinal epithelial cells of severe acute pancreatitis
rats receiving nutritional support in different ways

Ting Han, Xiuli Li, Donglian Cai, Yan Zhong, Lingyun Chen, Shanhan Geng, Shaojun Yin

Department of Clinical Nutrition, Shanghai 10th People’s Hospital, Tongji University School of Medicine, Shanghai 200072, China; Department
of Dermatology, Shanghai 10th People’s Hospital, Tongji University, Tongji University School of Medicine, Shanghai 200072, China;
Department of Clinical Nutrition, Changhai Hospital, Second Military Medical University, Shanghai 200433, China; Department of Gerontology,
Shanghai 10th People’s Hospital, Tongji University School of Medicine, Shanghai 200072, China

Received November 28, 2012; Accepted January 17, 2013; Epub February 15, 2013; Published March 1, 2013

Abstract: Objective: To investigate the effect of glutamine (Gln) on pro-inflammatory cytokines (TNF-α, IL-2 and IL-10) and the balance between
pro-inflammatory cytokines and anti-inflammatory cytokines in severe acute pancreatitis (SAP) rats receiving nutritional support in different
ways. Methods: Male SD rats (n=80) were randomly assigned into 5 groups: sham group, SAP+ parenteral nutrition (PN) group, SAP+ enteral
nutrition (EN) group, SAP+EN+Gln group and SAP+PN+Gln group. At the same time, rats in 5 groups were sacrificed at 4 and 7 days after
nutritional support. ELISA was employed to detect the pro-inflammatory cytokines including TNF-α, IL-2 and IL-10. Results: The serum TNF-α in
the EN+Gln group after 7-day treatment was significantly lower than that in the EN, PN and PN+Gln groups at corresponding time point (P<0.
05). The serum IL-2 in the EN+Gln group after 7-day treatment was markedly higher than that in the EN, PN and PN+Gln groups at
corresponding time point (P<0.01). After 7-day treatment, the serum IL-2 in the EN+Gln and EN groups were markedly higher than that after 4-
day treatment (P<0.01), but the serum IL-2 in the PN group was significantly lower than that after 4-day treatment (P<0.01). The serum IL-10
after 7-day treatment was markedly lower than that after 4-day treatment in all groups (P<0.01), and PN group had the lowest serum IL-10.
Serum IL-10 in the EN+Gln group was significantly higher than that in the PN and PN+Gln groups at both time points (P<0.01). The serum IL-10
in the EN group was significantly higher than that in the PN group after 4-day treatment (P<0.01), but the serum IL-10 in the EN group was
comparable to that in the PN group after 7-day treatment. The serum IL-10/TNF-α in the EN+Gln group was only slightly higher than that in the
control group at both time points. The serum IL-10/TNF-α in the EN group was significantly lower than that in the EN+Gln group at both time
points (P<0.05). The serum IL-10/TNF-α in the PN group was markedly lower than that in the EN group and EN+Gln group (P<0.05 and P<0.01,
respectively).Conclusion: EN in combination with Gln are superior to EN alone, PN alone and PN in combination with Gln in regulating
inflammation in SAP rats, and the EN has more potent capability to regulate the balance between pro-inflammation and anti-inflammation than
PN. (IJCEP1211039).

Keywords: Pancreatitis, glutamine, inflammatory cytokines, rat

Address correspondence to: Dr. Shaojun Yin, Department of Gerontology, Shanghai 10th People’s Hospital, Tongji University School of Medi-
cine, Shanghai 200072, China. Phone: 021-66301056; E-mail: blueisis@tongji.edu.cn; ying_yings200@163.com