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Int J Clin Exp Pathol 2013;6(3):421-430

Original Article
Therapeutic effect of 188Re-MAG3-depreotide on non-small cell lung cancer in vivo and
in vitro
 
Fei Yu, Mingli Lv, Haidong Cai, Dong Li, Xueyu Yuan, Zhongwei Lv

Department of Nuclear Medicine, Tenth People’s Hospital of Tongji University, Shanghai 200072, China

Received November 29, 2012; Accepted January 9, 2013; Epub February 15, 2013; Published March 1, 2013

Abstract: Objective: To investigate the in vivo and in vitro therapeutic effect of 188Re -MAG3-depreotide on non-small cell lung cancer (NSCLC).
Methods: MTT was done to measure the cell proliferation; flow cytometry to detect cell apoptosis; Transwell invasion assay to determine the
invasiveness of NSCLC. In addition, HE staining, TUNEL staining and immunohistochemistry for CD34 were employed to investigate the
influence of 188Re -MAG3-depreotide on the growth of NSCLC. Results: 1) Within 2-6 days, the inhibitory effect of 188Re -MAG3-depreotide on
the proliferation of A549 cells and SPC-A1 cells increased over time. 2) At 48 h after treatment with 188Re -MAG3-depreotide, the apoptosis rate
of A549 cells and SPC-A1 cells was 23.1% and 22.6%, respectively. 3) After 188Re -MAG3-depreotide treatment, the number of invasive A549
cells and SPC-A1 cells was reduced by about 3 times when compared with control group. 4) The cancer in the control group presented with
unlimited growth. The cancer growth continued after treatment with 188Re or MAG3-depreotide alone, while the cancer growth was markedly
inhibited after 188Re -MAG3-depreotide treatment when compared with control group. Conclusion: 188Re-MAG3-depreotide can inhibit the
proliferation and invasion of A549 cells and SPC-A1 cells. Treatment with 7.4MBq 188Re-MAG3-depreotide via tail vein can significantly
suppress the in vivo cancer growth and induce the apoptosis of cancer cells. These findings demonstrate that 188Re-MAG3-depreotide can
induce the apoptosis of NSCLC cells and directly kill the NSCLC cells, which provide evidence for the radiotherapy of NSCLC. (IJCEP1211041).

Keywords: 188Re, depreotide, non-small cell lung cancer, in vitro killing effect, cancer bearing nude mice

Address correspondence to: Dr. Zhongwei Lv, Department of Nuclear Medicine, Tenth People’s Hospital of Tongji University, Shanghai
200072, China. Phone: +8621-66301051; E-mail: yufei@tongji.edu.cn; doczhong@yeah.net