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Int J Clin Exp Pathol 2013;6(4):639-649

Original Article
Effects on high cholesterol-fed to liver, retina, hippocampus, and Harderian gland in
Goto-Kakizaki rat

Kanchana Kengkoom, Aekkarin Klinkhamhom, Aunchalee Sirimontaporn, Ornuma Singha, Taweesak Ketjareon, Yaowaluk Panavechkijkul,
Dutmanee Seriwatanachai, Suluck Ukong, Sumate Ampawong

Academic Services Office, Quality Assurance Office, Veterinary Medical Care Office, National Laboratory Animal Center, Mahidol University, 999
Salaya, Puttamonthon, Nakorn Pathom, 73170, Thailand; Department of Oral Medicine, Faculty of Dentistry, Mahidol University, Rajthevee,
Bangkok, 10400, Thailand; Department of Biology, Faculty of Science, Silpakorn University, Nakorn Pathom, 73000, Thailand

Received January 18, 2013; Accepted February 18, 2013; Epub March 15, 2013; Published April 1, 2013

Abstract: To understand the relationship among cholesterolemia, hyperglycemic stage in non obese type 2 diabetes mellitus, and histological
perturbations on liver, retina, hippocampus, and Harderian gland, we maintained rat on a diet high in cholesterol for fourteen weeks, then
analyzed blood lipid profiles, blood glucose, hepatic enzymes, and microscopic lesion of those tissues. We observed that high
cholesterol-treated rat elevated in cholesterol and low density lipoprotein with not correlated to hyperglycemia. Histopathological changing in
Goto-Kakizaki rat on liver (microvesicular steatosis) and Harderain gland (tubular lesions) were related to hyperglycemic effect rather than
cholesterolemic effect. These may be related to hypoinsulinemic characteristic of this diabetic model. However increasing pyknotic nuclei on
hippocampus and reducing of retinal ganglionic cell were related to the high level of cholesterol loaded with synergized effect due to diabetic
stage. (IJCEP1301036).

Keywords: High cholesterol, liver, retina, hippocampus, Harderian gland, Goto-Kakizaki rat

Address correspondence to: Dr. Sumate Ampawong, Veterinary Medical Care Office, National Laboratory Animal Center, Mahidol University,
999 Salaya, Puttamonthon, Nakorn-Pathom, 73170, Thailand. Tel: (662) 4419342 ext 114; Fax: (662) 4419342; E-mail:
am_sumate@hotmail.com