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Int J Clin Exp Pathol 2013;6(4):622-629

Original Article
Ferritin L and ferritin H are differentially located within hepatic and extra hepatic organs
under physiological and acute phase conditions

Shakil Ahmad, Federico Moriconi, Naila Naz, Sadaf Sultan, Nadeem Sheikh, Giuliano Ramadori, Ihtzaz Ahmed Malik

Department of Internal Medicine, Division of Gastroenterology and Endocrinology, University Hospital, Georg-August-University,
Rob-ert-Koch-Str. 40, 37075 Göttingen, Germany. These author contributed equally in the work.

Received January 23, 2013; Accepted February 21, 2013; Epub March 15, 2013; Published April 1, 2013

Abstract: Ferritin L (FTL) and Ferritin H (FTH) subunits are responsible for intercellular iron storage. We previously reported increasing
amounts of liver cytoplasmic and nuclear iron content during acute phase response (APR). Aim of the present study is to demonstrate
intracellular localization of ferritin subunits in liver compared with extra hepatic organs of rat under physiological and acute phase conditions.
Rats were administered turpentine-oil (TO) intramuscularly to induce a sterile abscess (acute-phase-model) and sacrificed at different time
points. Immunohistochemistry was performed utilizing horse-reddish-peroxidise conjugated secondary antibody on 4µm thick section. Liver
cytoplasmic and nuclear protein were used for Western blot analysis. By means of immunohistology, FTL was detected in cytoplasm while a
strong nuclear positivity for FTH was evident in the liver. Similarly, in heart, spleen and brain FTL was detected mainly in the cytoplasm while
FTH demonstrated intense nuclear and a weak cytoplasmic expression. Western blot analysis of cytoplasmic and nuclear fractions from liver,
heart, spleen and brain further confirmed mainly cytoplasmic expression of FTL in contrast to the nuclear and cytoplasmic expression of FTH.
The data presented demonstrate the differential localization of FTL and FTH within hepatic and extra hepatic organs being FTL predominantly in
the cytoplasm while FTH predominantly in nucleus. (IJCEP1301053).

Keywords: Ferritin, nuclear localization, liver, acute phase, iron regulation

Address correspondence to: Dr. Ihtzaz Ahmed Malik, Department of Internal Medicine, University Hospital Göttingen, Robert-Koch-Str. 40,
37075 Göttingen, Germany. Phone: 0049 (551) 398902; Fax: 0049 (551) 396921; E-mail: i.malik@med.uni-goettingen.de