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Int J Clin Exp Pathol 2013;6(4):669-677

Original Article
Upregulation of URI/RMP gene expression in cervical cancer by high-throughput tissue
microarray analysis

Junxia Gu, Xiaoyun Li, Yuting Liang, Longwei Qiao, Deyuan Ran, Yaojuan Lu, Xingang Li, Wenxiang Wei, Qiping Zheng

Department of Hematology and Hematological Laboratory Science, School of Medical Science and Laboratory Medicine, Jiangsu University,
Zhenjiang 212013, China; Department of Anatomy and Cell Biology, Rush University Medical Center, Chicago, IL 60612, USA; Department of
Hematology, Anyang District Hospital, Anyang 455000, China; Department of Cell Biology, School of Medicine, Soochow University, Suzhou
215123, China. These authors contribute equal to the paper.

Received February 17, 2013; Accepted March 7, 2013; Epub March 15, 2013; Published April 1, 2013

Abstract: URI, or RMP, is a RNA polymerase II subunit RPB5-associated protein known to play essential roles in ubiquitination and
transcrip-tion. Recently, we and others have shown that URI/RMP is also important for progression of hepatocellular carcinoma, ovarian, and
prostate cancers. To identify the mechanistic basis of URI/RMP during multiple cellular processes, we investigated URI/RMP expression in a
tissue microarray (TMA) containing multiple normal human tissues. The results showed that URI/RMP is ubiquitously but differentially
expressed in these human tissues which partially explains its multiple cellular functions. To elucidate the role of URI/RMP during oncogenesis
of multiple malignancies, especially the tumors of reproductive system, we analyzed URI/RMP expression in a TMA containing multiple
reproductive system tumors. We did not observe significant difference of URI/RMP expression between cancerous and adjacent tissues of the
prostate, breast, ovarian, and endometrial cancers. However, increased URI/RMP expression was observed in two of the three cases of
cervical SCC (squamous cell carcinoma) cells compared to their adjacent epithelial cells. Moreover, we detected significantly upregulated
URI/RMP expression not only in cervical cancers but also in pre-cancerous CINs (cervical intra-epithelial neoplasias) in a TMA that covers the
whole spectrum of normal cervix, CINs, and cervical cancers. No difference of URI/RMP expression was observed between CINs and cervical
cancers. Given the high risk of CINs (especially CIN3) turning into cervical cancer if left untreated, the increased URI/RMP expression in CINs
as well as in cervical cancers suggest a clinical relevance of URI/RMP upon cervical cancer tumorigenesis and worth further investigation.
(IJCEP1302019).

Keywords: URI/RMP, tissue microarray, immunohistochemistry, cervical cancer

Address correspondence to: Dr. Junxia Gu, Department of Hematology and Hematological Laboratory Science, School of Medical Science and
Laboratory Medicine, Jiangsu University, Zhenjiang 212013, China. Phone: 86-13812460959; E-mail: junxiagu@yahoo.com.cn or Dr. Qiping
Zheng, Department of Anatomy and Cell Biology, Rush University Medical Center, 600 South Paulina Street, Chicago, IL 60612. Phone:
1-312-942-5514; Fax: 1-312-942-5744; E-mail: qiping_zheng@rush.edu