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Int J Clin Exp Pathol 2013;6(5):821-830

Original Article
Effects of glycyl-glutamine dipeptide supplementation on myocardial damage and
cardiac function in rats after severe burn injury

Yong Zhang, Hong Yan, Shang-Gun Lv, Lin Wang, Guang-Ping Liang, Qian-Xue Wan, Xi Peng

State Key Laboratory of Trauma, Burns and Combined Injury, Institute of Burns of PLA, Southwest Hospital, Third Military Medical University,
Chongqing 400038, PR China. These authors contributed equally to this work.

Received March 6, 2013; Accepted April 3, 2013; Epub April 15, 2013; Published May 1, 2013

Abstract: Glutamine decreases myocardial damage in ischemia/reperfusion injury. However, the cardioprotective effect of glutamine after burn
injury remains unclear. Present study was to explore the protective effect of glycyl-glutamine dipeptide on myocardial damage in severe burn
rats. Seventy-two Wistar rats were randomly divided into three groups: normal control (C), burned control (B) and glycyl-glutamine
dipeptide-treated (GG) groups. B and GG groups were inflicted with 30% total body surface area of full thickness burn. The GG group was given
1.5 g/kg glycyl-glutamine dipeptide per day and the B group was given the same dose of alanine via intraperitoneal injection for 3 days. The
serum CK, LDH, AST, and, blood lactic acid levels, as well as the myocardium ATP and GSH contents, were measured. The indices of cardiac
contractile function and histopathological change were analyzed at 12, 24, 48, and 72 post-burn hours (PBH). The serum CK, LDH, AST and
blood lactic acid levels increased, and the myocardium ATP and GSH content decreased in both burned groups. Compared with B group, the
CK, LDH, AST and blood lactic acid levels reduced, myocardium ATP and GSH content increased in GG group. Moreover, the inhibition of
cardiac contractile function and myocardial histopathological damage were reduced significantly in GG group. We conclude that myocardial
histological structure and function were damaged significantly after burn injury, glycyl-glutamine dipeptide supplementation is beneficial to
myocardial preservation by improving cardiocyte energy metabolism, increasing ATP and glutathione synthesis. (IJCEP1303010).

Keywords: Glycyl-glutamine dipeptide, myocardial damage, energy metabolism, GSH, burns, rat

Address correspondence to: Dr. Xi Peng, State Key Laboratory of Trauma, Burns and Combined Injury, Institute of Burns of PLA, Southwest
Hospital, Third Military Medical University, Chongqing 400038, PR China. E-mail: pxlrmm@tmmu.edu.cn