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Int J Clin Exp Pathol 2013;6(6):1112-1120

Original Article
Determination of whole transcription profiles and specific pathways in invasive ductal
breast carcinoma

Pasra Arnutti, Manas Kotepui, Wichitra Asanprakit, Phaibul Punyarit, Porntip Chavalitshewinkoon-Petmitr, Talabporn Harnroongroj, Songsak
Petmitr

Department of Tropical Nutrition & Food Science, Faculty of Tropical Medicine, Mahidol University, Bangkok 10400, Thailand; Department of
Molecular Tropical Medicine & Genetics, Faculty of Tropical Medicine, Mahidol University, 420/6 Ratchawithi Road, Bangkok 10400, Thailand;
Department of Surgery, Phramongkutklao College of Medicine, Bangkok 10400, Thailand; Pathology Division, Army Institute of Pathology,
Bangkok 10400, Thailand; Department of Protozoology, Faculty of Tropical Medicine, Mahidol University, Bangkok 10400, Thailand. Present
address: Department of Pathology, Phramongkutklao College of Medicine, Bangkok 10400, Thailand.

Received March 11, 2013; Accepted March 26, 2013; Epub May 15, 2013; Published June 1, 2013

Abstract: Breast cancer is the most common cancer affecting women worldwide including Thailand. Whole transcription profiles of invasive
ductal breast carcinoma (IDC) obtained by oligonucleotide microarray should lead to a better understanding of the molecular basis of IDCs,
allow for examination of specific markers for diagnosis, and provide novel targets for therapy. This study aimed to detect the whole transcript
expression of approximately 35,000 target genes in Thai breast cancer patients, using Affymetrix GeneChip® Exon 1.0 Sense Target Arrays.
Analysis revealed that the differential expression profiles of 928 genes (423 up-regulated and 505 down-regulated genes) were 2-fold or
greater (unpaired t-test, p < 0.05) in invasive ductal breast cancer, compared with normal tissues. The Gene Ontology (GO) databases support
important associations in 17 gene sets with p-value < 1E-10 and ≥ 4-fold changes, involving the tumorigenic pathways of cell cycles,
extracellular regions, as well as cellular component organization. Likewise, the TGFBR and IL-6 pathways contain gene expression with
statistically significant changes in IDC. (IJCEP1303024).

Keywords: Invasive ductal breast carcinoma, oligonucleotide microarray, gene expression profile, TGFBR, IL-6 pathway

Address correspondence to: Dr. Songsak Petmitr, Department of Molecular Tropical Medicine & Genetics, Faculty of Tropical Medicine,
Mahidol University, 420/6 Ratchawithi Road, Bangkok 10400, Thailand. Tel: 662-3069138; Fax: 662-3069139; E-mail: songsak.pet@mahidol.ac.
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