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Int J Clin Exp Pathol 2013;6(5):917-925
Intestinal protein expression profile identifies inflammatory bowel disease and predicts
Jun Shen, Yuqi Qiao, Zhihua Ran, Tianrong Wang, Jiangtao Xu, Jinsun Feng
Division of Gastroenterology and Hepatology, Shanghai Jiao-Tong University School of Medicine Renji Hospital, Shanghai Institute of Digestive
Disease; Key Laboratory of Gastroenterology & Hepatology, Ministry of Health (Shanghai Jiao-Tong University), Shanghai, 200127, China;
National Engineering Center for Biochip at Shanghai, Shanghai, 201203, China
Received March 23, 2013; Accepted April 8, 2013; Epub April 15, 2013; Published May 1, 2013
Abstract: To date, most studies have applied individual factors as indicators of disease classification and prognosis. The aim of this study is
to determine whether clustering analysis of protein expression profiles in intestinal epithelia improves classification and prognosis in patients
with inflammatory bowel disease (IBD). One hundred and twenty Crohn’s disease (CD) patients, 117 ulcerative colitis (UC) patients and 120
cases of nonspecific colitis provided intestinal biopsy samples for tissue microarray (TMA). Both unsupervised and supervised analyses were
used for evaluation of clustering and association with relapse. There was a significant concordance between cluster groups based on
immunostaining data of TMA and clinical classification in distinguishing IBD from nonspecific colitis (kappa=0.498, p<0.001). CD27, CD70,
CD40, TRAF3, TRAF4 and TRAF2 presented similar immunostaining features, which were different from clusters of CD154, CD80 and TRAF5.
Moreover, higher expression of TRAF2 was a predictor of relapse in patients with UC (p=0.006).Thus, protein expression profiles can
distinguish IBD and nonspecific colitis, and combination analysis protein expression profiles show that TRAF2 can predict relapse of UC.
Keywords: Crohn’s disease, ulcerative colitis, classification, relapse
Address correspondence to: Dr. Zhi-hua Ran, Division of Gastroenterology and Hepatology, Shanghai Jiao-Tong University School of
Medicine Renji Hospital, Shanghai Institute of Digestive Disease; Key Laboratory of Gastroenterology & Hepatology, Ministry of Health
(Shanghai Jiao-Tong University), 1630# Dongfang Road, Shanghai 200127, P R China. Phone: +86 21 63260930; Fax: +86 21 63266027; E-