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Int J Clin Exp Pathol 2013;6(7):1337-1342

Original Article
Hyperbaric oxygen therapy provides neuroprotection following spinal cord injury in a rat
model

Huai Huang, Lei Xue, Xu Zhang, Qibiao Weng, Huiqiang Chen, Jing Gu, Shuilin Ye, Xiaodong Chen, Wei Zhang, Huangyi Liao

Neurological Rehabilitation, Division II. Neurology Specialty Hospital, Guangzhou General Hospital of Guangzhou Military Command,
Guangzhou 510010, Guangdong Province, China; Center of HBOT, Affiliated Nanfang Hospital of Southern Medical University, Guangzhou
510515, China; Department of Hyperbaric Oxygen Therapy, Zhujiang Hospital of Southern Medical University, Guangzhou 510000, China;
Department of Orthopedics, Guangzhou General Hospital of Guangzhou Military Command, Guangzhou 510010, China; Department of
Pathology, Guangzhou General Hospital of Guangzhou Military Command, Guangzhou 510010, China; Department of Hyperbaric Oxygen
Therapy, Xinhui People Hospital of Jiangmen, Jiangmen 529100, China

Received April 10, 2013; Accepted May 17, 2013; Epub June 15, 2013; Published July 1, 2013

Abstract: Objective: To investigate the effect of hyperbaric oxygen therapy (HBOT) on the iNOS mRNA-iNOS-NO signaling pathway and
neurofunction protected in a rat spinal cord injury model. Methods: A total of 36 Sprague-Dawley rats were randomly divided into 3 groups:
control group (n=12), SCI group (n=12) and SCI + HBOT group (n=12). SCI + HBOT group In the SCI group and SCI + HBOT groups, SCI was
performed on rats. In the SCI + HBOT group, rats with SCI underwent HBO treatment 30 min after SCI for 24 sessions. After HBO therapy,
measurement of motor evoked potential (MEP), Basso, Beattie, Bresnahan (BBB) scoring and pathological examination were done. RT-PCR
and immunohistochemistry were employed to detect the mRNA and protein expression of iNOS, respectively. Diazo colorimetry was performed
to detect the serum NO content. Results: The mRNA and protein expression of iNOS in the spinal cord and the serum NO content were
markedly increased in the SCI group as compared to the control group (P<0.05). However, the mRNA and protein expression of iNOS and the
serum NO content were dramatically reduced in the SCI + HBOT group as compared to the SCI group (P<0.05). Conclusion: HBO therapy can
promote the neuroprotection following SCI, which may be related to the effect of HBO on the iNOS mRNA-iNOS-NO signaling pathway.
(IJCEP1304013).

Keywords: Hyperbaric oxygen, spinal cord injury, rat, nitric oxide, inducible nitric oxide synthase

Address correspondence to: Dr. Huai Huang, Neurological Rehabilitation, Division II. Neurology Specialty Hospital, Guangzhou General
Hospital of Guangzhou Military Command 510010, Guangdong Province, China. E-mail: huanghuai1999@163.com; huanghbot@163.com