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Int J Clin Exp Pathol 2013;6(7):1409-1415

Original Article
HPV frequency in penile carcinoma of Mexican patients: important contribution of HPV16
European variant

Ricardo López-Romero, Candela Iglesias-Chiesa, Brenda Alatorre, Karla Vázquez, Patricia Piña-Sánchez, Isabel Alvarado, Minerva Lazos, Raúl
Peralta, Beatriz González-Yebra, Ana E Romero, Mauricio Salcedo

Laboratorio de Oncología Genómica, Unidad de Investigación Médica en Enfermedades Oncológicas, Hospital de Oncología, Centro Médico
Nacional Siglo XXI, Instituto Mexicano del Seguro Social, México D.F.; Servicio de Anatomía Patológica, Hospital de Oncología, Centro Médico
Nacional Siglo XXI, Instituto Mexicano del Seguro Social, México D.F.; Departamento de Patología, Hospital General de México SS, México D.F.;
Departamento de Matemáticas, Facultad de Ciencias, UNAM, México, D.F.; Hospital Regional de Alta Especialidad del Bajío, IMSS, León
Guanajuato, México; Departamento de Medicina y Nutrición, División Ciencias de la Salud, Universidad de Guanajuato Campus León, León,
Gto. México

Received April 29, 2013; Accepted May 24, 2013; Epub June 15, 2013; Published July 1, 2013

Abstract: The role of human papillomavirus (HPV) infection in penile carcinoma (PeC) is currently reported and about half of the PeC is
associated with HPV16 and 18. We used a PCR-based strategy by using HPV general primers to analyze 86 penile carcinomas
paraffin-embedded tissues. Some clinical data, the histological subtype, growth pattern, and differentiation degree were also collected. The
amplified fragments were then sequenced to confirm the HPV type and for HPV16/18 variants. DNA samples were also subjected to relative
real time PCR for hTERC gene copy number. Some clinical data were also collected. Global HPV frequency was 77.9%. Relative contributions
was for HPV16 (85%), 31 (4.4%), 11 (4.4%), 58, 33, 18, and 59 (1.4% each one). Sequence analysis of HPV16 identified European variants and
Asian-American (AAb-c) variants in 92% and in 8% of the samples, respectively.  Furthermore hTERC gene amplification was observed in only
17% of the cases. Our results suggest that some members of HPV A9 group (represented by HPV16, 58, and 31) are the most frequent among
PeC patients studied with an important contribution from HPV16 European variant. The hTERC gene amplification could be poorly related to
penile epithelial tissue. (IJCEP1304039).

Keywords: HPV, penile carcinoma, México

Address correspondence to: Dr. Salcedo Mauricio, Laboratorio de Oncología Genómica, Unidad de Investigación Médica en Enfermedades
Oncológicas, Hospital de Oncología, CMN SXXI-IMSS. Av. Cuauhtémoc 330, Col. Doctores, México, Distrito Federal 06720, México. Tel:
55566276900 Ext. 22706; E-mail: maosal89@yahoo.com