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Int J Clin Exp Pathol 2013;6(7):1272-1281

Original Article
Neuro-invasion of Chandipura virus mediates pathogenesis in experimentally infected
mice

Balakrishnan Anukumar, Balasubramaniam G Amirthalingam, Vijay N Shelke, Rashmi Gunjikar, Poonam Shewale

National Institute of Virology Kerala unit, Govt. T. D. Medical college hospital, Vandanam, Alappuzha district, Kerala, India, Zip code: 688005;
Department of pathology, Veterinary College and Research Institute, Namakkal, Tamil Nadu, India; Electron microscope & Histopathology
group, National institute of Virology, Pune-1; Encephalitis Group, Microbial containment complex, National institute of Virology, Pashan;
Chandipura virus group, National institute of Virology, Pune-1

Received May 7, 2013; Accepted May 22, 2013; Epub June 15, 2013; Published July 1, 2013

Abstract: Neuro-tropism is a major feature in many viral infections. Chandipura virus produces neurological symptoms in naturally infected
young children and experimentally infected suckling mice. This study was undertaken to find out the neuro-invasive behaviour of Chandipura
virus in suckling mice. The suckling mice were infected with the virus via footpad injection. Different tissues were collected at 24-h intervals up
to 96-h post infection and processed for virus quantification and histological study. Further confirming the virus predilection to nerves tissues,
the adult mice were inoculated with the virus via different routes. The suckling mice experimental results revealed a progressive replication of
virus in spinal cord and brain. The progressive-virus replication was not observed in the other tissues like kidney, spleen, liver etc.
Histo-pathological lesions noticed in the spinal cord and brain tissues suggested the extensive damages in these tissues. In adult mice
experiment, the virus replication observed only in the brain of the mice infected via intra-cerebral route. From this study, we conclude that
nervous tissues are predilection sites for Chandipura virus replication in suckling and adult mice. In suckling mice, virus might transmit
through nervous tissues for dissemination. In contrast, the adult mice the nervous terminal might not pick up the virus through footpad infection.
The pathogenesis in mice might be due to the virus replication mediated damage in the central nervous system. (IJCEP1305004).

Keywords: Chandipura virus, axonal transport, neuro tropic viruses, neuro-pathogenesis

Address correspondence to: Dr. Balakrishnan Anukumar, National Institute of Virology Kerala unit, Govt. T. D. Medical College hospital,
Vandanam, Alappuzha, Kerala state, India, Zip code: 688005. Phone: 914772280500; Fax: 914772280100; E-mail: anukumar@icmr.org.in;
anukumar_b74@yahoo.com