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Int J Clin Exp Pathol 2013;6(7):1351-1361

Original Article
Gene and microRNA expression reveals sensitivity to paclitaxel in laryngeal cancer cell
line

Cheng-Zhi Xu, Jin Xie, Bin Jin, Xin-Wei Chen, Zhen-Feng Sun, Bao-Xing Wang, Pin Dong

Department of Otolaryngology-Head and Neck Surgery, Shanghai First People’s Hospital affiliated to Shanghai Jiao Tong University, Shanghai,
China; Department of Otolaryngology-Head and Neck Surgery, Shanghai Ninth People’s Hospital, School of medicine, Shanghai Jiao Tong
University, Shanghai, China

Received May 7, 2013; Accepted May 29, 2013; Epub June 15, 2013; Published July 1, 2013

Abstract: Paclitaxel is a widely used chemotherapy drug for advanced laryngeal cancer patients. However, the fact that there are 20-40% of
advanced laryngeal cancer patients do not response to paclitaxel makes it necessary to figure out potential biomarkers for paclitaxel sensitivity
prediction. In this work, Hep2, a laryngeal cancer cell line, untreated or treated with lower dose of paclitaxel for 24 h, was applied to DNA
microarray chips for gene and miR expression profile analysis. Expression of eight genes altered significantly following paclitaxel treatment,
which was further validated by quantitative real-time PCR. Four up-regulated genes were ID2, BMP4, CCL4 and ACTG2, in which ID2 and BMP4
were implicated to be involved in several drugs sensitivity. While the down-regulated four genes, MAPK4, FASN, INSIG1 and SCD, were mainly
linked to the endoplasmic reticulum and fatty acid biosynthesis, these two cell processes that are associated with drug sensitivity by increasing
evidences. After paclitaxel treatment, expression of 49 miRs was significantly altered. Within these miRs, the most markedly expression-
changed were miR-31-star, miR-1264, miR-3150b-5p and miR-210. While the miRs putatively modulated the mRNA expression of the most
significantly expression-altered genes were miR-1264, miR-130a, miR-27b, miR-195, miR-1291, miR-214, miR-1277 and miR-1265, which
were obtained by miR target prediction and miRNA target correlation. Collectively, our study might provide potential biomarkers for paclitaxel
sensitivity prediction and drug resistance targets in laryngeal cancer patients. (IJCEP1305005).

Keywords: Laryngeal cancer, paclitaxel, gene expression profiles, miR expression profiles, cell lines

Address correspondence to: Pin Dong, Department of Otolaryngology-Head and Neck Surgery, Shanghai First People’s Hospital affiliated to
Shanghai Jiao Tong University, 85 Wujin Road, Shanghai, 200080, China. E-mail: dongpin_sh@163.com