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Int J Clin Exp Pathol 2013;6(8):1603-1609

Original Article
In vitro apoptosis effects of GnRHII on endometrial stromal cells from patients with
endometriosis

Fengying Huang, Ying Zou, Huanping Wang, Jing Cao, Tuanfang Yin

Department of Obstetrics and Gynecology, Second Xiangya Hospital, Central South University, Changsha, Hunan, China, 410011; Department
of Otorhinolaryngology, Second Xiangya Hospital, Central South University, Changsha, Hunan, China, 410011

Received May 18, 2013; Accepted June 17, 2013; Epub July 15, 2013; Published August 1, 2013

Abstract: Purpose: To study the effect of gonadotropin-releasing hormone II (GnRHII) on the cell apoptosis of ectopic, eutopic and normal
endometrial stromal cells cultured in vitro from endometriosis patients, and to provide theoretical basis for exploring new treatments for
endometriosis (EMs). Methods: Ectopic, eutopic and normal endometrial stromal cells were isolated, cultured and identified in vitro, then
treated with different concentrations of GnRHII (0, 10-10 M, 10-8 M and 10-6 M). Cell apoptosis was detected by Hoechst staining and flow
cytometry. Results: GnRHII increased apoptosis in ectopic, eutopic and normal stromal cells in a dosage-dependent manner (P<0.05), and
apoptosis of ectopic stroma cells was significantly higher than that of eutopic and normal cells (P<0.05); apoptosis in eutopic and normal cells
had no different (P>0.05). Conclusion: GnRHII can significantly induce apoptosis in ectopic, eutopic and normal endometrial stromal cells from
patients with endometriosis, especially to the ectopic. (IJCEP1305026).

Keywords: Endometriosis (EMs), gonadotropin-releasing hormone II (GnRHII), apoptosis, endometrial stromal cell (ESC), cell culture, in vitro

Address correspondence to: Dr. Tuanfang Yin, Department of Otorhinolaryngology, Second Xiangya Hospital, Central South University, 139#,
Renmin Road, Changsha, Hunan, China, 410011. Phone: +8613874870794; E-mail: ytfyin@163.com; hfy6697@163.com