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Int J Clin Exp Pathol 2013;6(8):1610-1616
Sperm protein 17, MAGE-C1 and NY-ESO-1 in hepatocellular carcinoma: expression
frequency and their correlation with clinical parameters
Qiu-Yuan Xia, Song Liu, Fang-Qiu Li, Wen-Bin Huang, Li-Ning Shi, Xiao-Jun Zhou
Department of Pathology, Jinling Hospital, Nanjing University School of Medicine, Nanjing, Jiangsu 210002, China; Laboratory of Molecular
Biology, Institute of Medical Laboratory Sciences, Jinling Hospital, School of Medicine, Nanjing University, Nanjing Jiangsu 210002, China;
Department of General Surgery, Jinling Hospital, School of Medicine, Nanjing University, Nanjing, Jiangsu 210002, China; Department of
Pathology, Nanjing First Hospital Affiliated to Nanjing Medical University, Nanjing, Jiangsu 210006, China. Authors contributed equally.
Received June 3, 2013; Accepted June 18, 2013; Epub July 15, 2013; Published August 1, 2013
Abstract: This study is dedicated to investigate the expression patterns of sperm protein 17 (Sp17), melanoma-specific antigen (MAGE)-C1
and New York esophageal squamous cell carcinoma-1 (NY-ESO-1), to explore the correlation between these cancer-testis antigens and
clinical parameters, and to evaluate their values in diagnosis and differentiation of hepatocellular carcinoma. Methods: Immunohistochemical
staining was performed in 45 paraffin-embedded hepatocellular carcinoma specimens. 45 normal peripheral hepatic tissues collected from
adjacent non-cancerous areas were used as controls. Results: Positive results of immunohistostaining were obtained in 16 (35.6%), 7
(15.6%) and 36 (80.0%) samples using MAGE-C1, NY-ESO-1 and Sp17 antibodies, respectively. The immunoreactivity of Sp17 was also found
in 7 (14.0%) control samples. A statistical correlation between the frequency of Sp17 expression and tumor differentiation grade in
hepatocellular carcinoma was confirmed. Conclusions: Sp17 is highly expressed in hepatocellular carcinoma cells. The frequency of Sp17
expression is closely related to the pathologic differentiation in hepatocellular carcinoma. (IJCEP1306001).
Keywords: Sp17, MAGE-C1, NY-ESO-1, cancer-testis antigens, hepatocellular carcinoma
Address correspondence to: Dr. Xiao-Jun Zhou, Department of Pathology, Jinling Hospital, Nanjing University School of Medicine, Nanjing,
Jiangsu 210002, China. Phone: +86-13851695108; E-mail: email@example.com and Dr. Fang-qiu Li, Laboratory of Molecular Biology, Institute of
Medical Laboratory Sciences, Jinling Hospital, Nanjing University School of Medicine, Nanjing Jiangsu, 210002, China. Phone:
+86-13801583645; E-mail: firstname.lastname@example.org