IJCEP Copyright © 2007-All rights reserved.
Int J Clin Exp Pathol 2013;6(8):1532-1537

Original Article
Histometric analyses of cancellous and cortical interface in autogenous bone grafting

Henrique Duque Netto, Sergio Olate, Leandro Klüppel, Antonio Marcio Resende do Carmo, Bélgica Vásquez, Jose Albergaria-Barbosa

Dental School, Federal University of Juiz de Fora, Juiz de Fora, Brazil; Dental School, Universidad de La Frontera, Temuco, Chile; Center for
Biomedical Research, Universidad Autónoma de Chile, Chile; Dental School, Federal University of Ponta Grossa, Brazil; Univeersidad de
Tarapacá, Chile; Piracicaba Dental School, State University of Campinas, Piracicaba, Brazil

Received June 18, 2013; Accepted July 7, 2013; Epub July 15, 2013; Published August 1, 2013

Abstract: Surgical procedures involving the rehabilitation of the maxillofacial region frequently require bone grafts; the aim of this research was
to evaluate the interface between recipient and graft with cortical or cancellous contact. 6 adult beagle dogs with 15 kg weight were included in
the study. Under general anesthesia, an 8 mm diameter block was obtained from parietal bone of each animal and was put on the frontal bone
with a 12 mm 1.5 screws. Was used the lag screw technique from better contact between the recipient and graft. 3-week and 6-week
euthanized period were chosen for histometric evaluation. Hematoxylin-eosin was used in a histologic routine technique and
histomorphometry was realized with IMAGEJ software. T test was used for data analyses with p<0.05 for statistical significance. The result
show some differences in descriptive histology but non statistical differences in the interface between cortical or cancellous bone at 3 or 6
week; as natural, after 6 week of surgery, bone integration was better and statistically superior to 3-week analyses. We conclude that
integration of cortical or cancellous bone can be usefully without differences. (IJCEP1306014).

Keywords: Bone defects, bone grafting, animal study

Address correspondence to: Dr. Sergio Olate, Division of Oral and Maxillofacial Surgery, School of Medicine, University of La Frontera, Claro
Solar No 115, Temuco, Chile. Phone: 56-45-325000; E-mail: sergio.olate@ufrontera.cl