IJCEP Copyright © 2007-All rights reserved.
Int J Clin Exp Pathol 2013;6(9):1861-1867

Original Article
Immunochemiluminescent detection of galectin-3 in tumoral tissue from prostate

Jorge Luiz Araújo-Filho, Mário Ribeiro Melo-Junior, Eduardo Isidoro Carneiro Beltrão, Luiza Rayanna Amorim de Lima, Consuelo Barreto Lins
Antunes, Luiz Bezerra de Carvalho Jr

Laboratório de Imunopatologia Keizo Asami, LIKA/UFPE, Recife, Pernambuco, 7851, Brazil; Departamento de Patologia, CCS/UFPE, Recife,
Pernambuco, 7851, Brazil; Departamento de Bioquímica, CCB/UFPE, Recife, Pernambuco, 7851, Brazil; Hospital Universitário Osvaldo Cruz,
HUOC/UPE, Recife, Pernambuco, 3447, Brazil

Received June 24, 2013; Accepted July 23, 2013; Epub August 15, 2013; Published September 1, 2013

Abstract: This work proposes a chemiluminescent quantitative method for galectin-3 (Gal3) detection in prostate tissues. Monoclonal antibody
anti-Gal3 was conjugated to acridinium ester (AE) and the complex formed with Gal3 in the prostate tissue was chemiluminescently detected.
The light emission (expressed in Relative Light Unit-RLU) showed mean values higher for benign prostatic hyperplasia than normal tissues
and adenocarcinoma. These differences showed to be statistically significant (p < 0.001). There was a linear relationship between RLU and
tissue area. Furthermore, these values were dramatically reduced when the tissue samples were previously incubated with non labeled
anti-Gal3. Finally, the anti-Gal3-AE solution in buffer stored at 4°C and the treated samples showed to be stable during a year and at least 72 h,
respectively. Gal3 content in prostate tissue was higher in benign prostatic hyperplasia than normal tissues and much lower in
adenocarcinoma. This quantitative, specific and sensitive method based on labeling antibody to acridinium ester can be applied to detect
antigen in tissue. (IJCEP1306025).

Keywords: Chemiluminescence, galectin-3, prostate tissue, immunohistochemistry

Address correspondence to: Dr. Luiz Bezerra de Carvalho Júnior, Departamento de Bioquímca e Laboratório de Imunopatologia Keizo Asami.
Av. Prof. Moraes Rego S/N, Cidade Universitária, Recife/PE, Brazil, 7851. Tel: +55 81 2126 8484; Fax: +55 81 2126 8485; E-mail:
lbcj@hotlink.com.br