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Int J Clin Exp Pathol 2013;6(9):1702-1712

Original Article
Sinomenine protects mice against ischemia reperfusion induced renal injury by
attenuating inflammatory response and tubular cell apoptosis

Zhiqing Zhao, Rui Guan, Shaohua Song, Mingjian Zhang, Fang Liu, Meng Guo, Wenyuan Guo, Qilin Yu, Luding Zhang, Quanxing Wang

Changhai Hospital, Second Military Medical University, Shanghai 200003, China; Institute of Organ Transplantation, Changzheng Hospital,
Second Military Medical University, Shanghai 200003, China; National Key Laboratory of Medical Immunology and Department of Immunology,
Second Military Medical University, Shanghai 200433, China; The Center for Biomedical Research, Tongji Hospital, Tongji Medical College,
Huazhong University of Science and Technology, Wuhan 430030, China. These authors contributed equally to this work.

Received July 2, 2013; Accepted August 1, 2013; Epub August 15, 2013; Published September 1, 2013

Abstract: Sinomenine (SIN) is a purified alkaloid from the Chinese herb Sinomenium acutum. Previous studies demonstrated that SIN
possesses anti-inflammatory and anti-apoptotic properties. We thus in the present report conducted studies to examine its impact on ischemia
reperfusion (IR) induced renal injury. Precondition of mice with 200 mg/kg of SIN provided significant protection for mice against IR-induced
renal injury as manifested by the attenuated serum creatinine (Cre) and blood urea nitrogen (BUN) along with less severity for histological
changes and tubular cell apoptosis. In line with these results, treatment of mice with SIN suppressed IR-induced inflammatory infiltration and
the expression of chemokine CXCL-10, adhesion molecule ICAM-1, and cytokines TNF-а/IL-6. Mechanistic studies revealed that SIN inhibits
NF-κB transcriptional activity to suppress IR-induced inflammatory response in the kidney, while it attenuates MAP kinase signaling to prevent
tubular cells undergoing apoptosis after IR insult. Altogether, our data support that SIN could be a useful therapeutic agent for prevention and
treatment of IR-induced renal injury in the clinical settings. (IJCEP1307005).

Keywords: Sinomenine, reperfusion injury, inflammation, apoptosis

Address correspondence to: Dr. Quanxing Wang, National Key Laboratory of Medical Immunology and Department of Immunology, Second
Military Medical University, 800 Xiangyin Rd, Shanghai 200433, China. Tel: +86-21-81871006; Fax: +86-21-81871006; E-mail:
wangqx64@hotmail.com; Dr. Luding Zhang, Institute of Organ Transplantation, Changzheng Hospital, Second Military Medical University, 415
Fengyang Road, Shanghai 200003, China. Tel: +86-21-81886668; Fax: +86-21- 81886668; E-mail: zhangld2010@163.com