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Int J Clin Exp Pathol 2013;6(11):2487-2496

Original Article
Effect of GnRH-II on the ESC proliferation, apoptosis and VEGF secretion in patients with
endometriosis in vitro

Fengying Huang, Huanping Wang, Ying Zou, Qiuhong Liu, Jing Cao, Tuanfang Yin

Departments of Obstetrics and Gynecology and Otorhinolaryngology, Second Xiangya Hospital, Central South University, Changsha, Hunan,
China, 410011; Department of Obstetrics and Gynecology, Henan Province People’s Hospital, Zhengzhou, Henan, China, 450000; Department
of Obstetrics and Gynecology, Hunan Province Maternity and Children’s Health Care Center, Changsha, Hunan, China, 410008; Department of
Obstetrics and Gynecology, Hunan Province People’s Hospital, Changsha, Hunan, China, 410002

Received July 4, 2013; Accepted August 7, 2013; Epub October 15, 2013; Published November 1, 2013

Abstract: Objective: To study the effect of GnRH-II on the cell proliferation, apoptosis and secreting vascular endothelial growth factor (VEGF) of
ectopic, eutopic and normal endometrial stromal cells (ESC) from patients with or without endometriosis (EMs) in vitro. Methods: The ectopic,
eutopic and normal ESC were isolated, cultured and identified, then added 0 M, 10-10 M, 10-8 M, 10-6 M GnRH-II. The growth and proliferation
of three ESC were measured by MTT assay; the cell apoptosis were detected with the method of Hoechst staining and Flow Cytometry test;
ELISA was used to measure the VEGF concentration change by three ESC secretion. Results: GnRH-II inhibited the proliferation of ectopic,
eutopic ESC from patients with endometriosis and normal ESC from control patients, in a dose- and time-dependent manner (P<0.05); GnRH-
II increased the apoptotic rate of three ESC in a dose-dependent manner (P<0.05); The concentration of VEGF in three ESC was significantly
decreased after the treatment of GnRH-II, in a dose-dependent manner (P<0.01); And these above effects were the strongest on the ectopic
than on the eutopic or normal, there were statistical significance (P<0.05); and three was no significantly difference between the eutopic and
normal (P>0.05). Conclusions: GnRH-II significantly inhibited the cell proliferation, induced cell apoptosis and decreased the VEGF secreting of
ectopic, eutopic and normal ESC in EMs in vitro, and these effects were the strongest on ectopic ESC, which suggested that GnRH-II may
become a new effective treatment for endometriosis. (IJCEP1307006).

Keywords: Endometriosis (EMs), gonadotropin releasing hormone-II (GnRH-II), endometrial stromal cells (ESC), cell proliferation, cell
apoptosis, vascular endothelial growth factor (VEGF), in vitro

Address correspondence to: Dr. Tuanfang Yin or Fengying Huang, Departments of Obstetrics and Gynecology and Otorhinolaryngology,
Second Xiangya Hospital, Central South University, Changsha, Hunan, China, 410011. Tel: +8613874870794; E-mail: ytfyin@163.com (TFY);
hfy6697@163.com (FYH)