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Int J Clin Exp Pathol 2013;6(9):1854-1860

Original Article
Increased expression of EphA1 protein in prostate cancers correlates with high Gleason
score

Libo Peng, Haiyan Wang, Yingchun Dong, Jie Ma, Juanjuan Wen, Jinrong Wu, Xueqing Wang, Xiaojun Zhou, Jiandong Wang

Southern Medical University, Guangzhou, China; Clinical Medical School of Southern Medical University, Nanjing, China; Department of
Pathology, Jinling Hospital, Nanjing University School of Medicine, Nanjing, China

Received July 18, 2013; Accepted August 12, 2013; Epub August 15, 2013; Published September 1, 2013

Abstract: The erythropoietin-producing hepatocellular (Eph) family of receptor tyrosine kinases regulates a multitude of physiological and
pathological processes. EphA1 is the first member of Eph superfamily and is involved in carcinogenesis. The aim of this study was to
investigate the expression of EphA1 in prostate cancers cell lines and the tissues, then explore the correlation with the clinicopathologic
parameters. The EphA1 transcript expression in prostate cancer cell lines was detected by Quantitative real-time PCR. The expression of
EphA1 protein in 138 prostate cancer tissue samples and 21 benign prostate hyperplasia samples were checked by using
immunohistochemical staining. EphA1 mRNA was high expressed in LNCap, moderately expressed in 22RV1 and Du145, and lost in PC3.
Loss of expression of EphA1 transcript was related to hypermethylation of CpG island around the translation start site. EphA1 protein was
differentially expressed in prostate cancers and hyperplasia. Increased expression of EphA1 protein was more frequently detected in prostate
cancers than in hyperplasia (P = 0.02), and more often detected in prostate cancer with high Gleason score (P < 0.001). Our data indicate that
EphA1 receptor may have roles in carcinogenesis and progression of prostate cancer, and can be a potentially useful target for prognostic and
therapeutic application. (IJCEP1307032).

Keywords: Prostate cancer, EphA1, Quantitative real-time PCR, immunohistochemistry

Address correspondence to: Dr. Jiandong Wang, Clinical Medical School of Southern Medical University, Department of Pathology, Jinling
Hospital, Nanjing University School of Medicine, Nanjing, 210002, China. Tel: +86-25-80860192; Fax: +86-25-80860191; E-mail:
jiandongwang1216@163.com