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Int J Clin Exp Pathol 2013;6(10):2048-2055

Original Article
Clinicopathological study of gene rearrangement and microRNA expression of primary
central nervous system diffuse large B-cell lymphomas

Jinfeng Zheng, Jiagang Xu, Shufang Ma, Xiyan Sun, Ming Geng, Lin Wang

Department of Pathology, The General Hospital, Jinan Military Command, Jinan, China, 250031; Department of Medical Supply, The 89th
Hospital of PLA, Weifang, China, 261021; Department of Community, Jinan No. 5 People’s Hospital, Jinan, Shandong, 250022; Department of
Neurosurgery, The General Hospital, Jinan Military Command, Jinan, China, 250031; Department of Laboratory Medicine, The General
Hospital, Jinan Military Command, Jinan, China, 250031. Equal contributors.

Received July 21, 2013; Accepted August 18, 2013; Epub September 15, 2013; Published October 1, 2013

Abstract: We studied the clinicopathological and imaging characteristics of primary central nervous system diffuse large B-cell lymphomas
(PCNS-DLBCL). Imaging, pathologic histology, and immunohistochemical staining characteristics were analyzed, and the immunoglobulin
heavy and light chain gene rearrangement of 25 PCNS-DLBCL cases was examined. MicroRNA was extracted from 10 cases each of PCNS-
DLBCL, extracerebral germinal center DLBCL (GC-DLBCL), and extracerebral non-GC-DLBCL (NGC-DLBCL); we conducted chip hybridization
and comparatively analyzed the difference among the three. PCNS-DLBCLs typically involved no less than two cerebral lobes (10/25); the
frontal lobe was affected most often (6/25). Target-shaped structures were observed in all PCNS-DLBCLs due to the proliferation of centroblast-
like large lymphocytes surrounding the vessels. There was strong and diffuse immunostaining for CD20 and CD79a, and negative
immunostaining for CD3, CD5, CD23, and cyclin D1 for all PCNS-DLBCLs. The percentage of cells with nuclear positivity for anti-Ki67 antibody
ranged 50-90% (mean, 80%). Three, 19, and 22 PCNS-DLBCLs were CD10-, Bcl-6-, and melanoma ubiquitous mutated 1-positive,
respectively. Twenty-four PCNS-DLBCLs were B-cell monoclonal. MicroRNA hybridization showed that 788 PCNS-DLBCL
microRNAs/segments increased to at least twice that of NGC-DLBCLs, and 401 PCNS-DLBCL microRNAs/segments declined to less than
half of that of NGC-DLBCLs. Six hundred and eleven PCNS-DLBCL microRNAs/segments increased to at least twice that of GC-DLBCLs, and
229 PCNS-DLBCL microRNAs/segments declined to less than half of that in GC-DLBCLs. PCNS-DLBCL typically affected multiple sites,
tended to occur in older men, arose from activated B cells, had high B-cell monoclonality; its microRNA expression differed from that of NGC-
DLBCL and GC-DLBCL. (IJCEP1307035).

Keywords: Central nervous system, diffuse large B-cell lymphoma, gene rearrangement, microRNA

Address correspondence to: Dr. Ming Geng and Lin Wang, Department of Pathology and Department of  Laboratory Medicine, The General
Hospital, Jinan Military Command, Shifan Road 25, Jinan, Shandong Province, China, 250031. Tel: +86 531 51666857; Fax: +86 531
51666857; E­-mail: gm2227@sina.com (Ming Geng); bqlchina@yahoo.com (Lin Wang)