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Int J Clin Exp Pathol 2013;6(10):2039-2047

Original Article
Functional regeneration of irradiated salivary glands with human amniotic epithelial cells
transplantation

Ni-Ni Zhang, Gui-Lin Huang, Qing-Bin Han, Xiaohua Hu, Jie Yi, Li Yao, Yuanchun He

Department of Oral and Maxillofacial Surgery, Stomatological Hospital, ZunYi Medical College, ZunYi 563003, China; Department of
Stomatology, LinYi People’s Hospital, LinYi 276003, China; Department of Stomatology, HanZhong People’s Hospital, LinYi 276003, China

Received July 30, 2013; Accepted August 19, 2013; Epub September 15, 2013; Published October 1, 2013

Abstract: This study aimed to investigate the functional restoration of radiation-damaged salivary gland with human amniotic epithelial cells
(hAECs) transplantation by intra-glandular injection. hAECs were isolated from the amnion tissues. After primary culture, the phenotype of
hAECs of the second passage was identified by flow cytometry (FCM) and immunocytochemical staining. Then, hAECs were intra-glandularly
injected into the irradiated glands of mice. At different time points after transplantation, the glands were collected for hematoxylin-eosin (HE)
staining and immunofluorescence staining, and the saliva flow rate was also determined. Results showed these cells were positive for CD29,
CD73 and CK19 and negative for CD44, CD34, CD45 and CD71. The transplanted hAECs in the recipient glands could differentiate into acinar-
like cells and resulted in morphological and functional restoration of salivary gland. (IJCEP1307048).

Keywords: Functional regeneration, salivary gland, irradiated, amniotic epithelial cells

Address correspondence to: Gui-Lin Huang, Department of Oral and Maxillofacial Surgery, Stomatological Hospital, ZunYi Medical College,
ZunYi 563003, China. E-mail: huang898988@sina.com; Qing-Bin Han, Department of Stomatology, LinYi People’s Hospital, LinYi 276003,
China. E-mail: han198502@126.com