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Int J Clin Exp Pathol 2013;6(10):2064-2070

Original Article
Upregulation of ATF-3 is correlated with prognosis and proliferation of laryngeal cancer
by regulating Cyclin D1 expression

Jiapeng Feng, Qingfeng Sun, Tianyi Wu, Jianguang Lu, Lingmei Qu, Yanan Sun, Linli Tian, Binghui Zhang, Dandan Li, Ming Liu

Department of Otorhinolaryngology, Head and Neck Surgery, Department of General Surgery, The Second Affiliated Hospital, Harbin Medical
University, Harbin, China. Equal contributors.

Received July 31, 2013; Accepted August 21, 2013; Epub September 15, 2013; Published October 1, 2013

Abstract: Objective: This study aimed to investigate the expression and significance of ATF-3 in laryngeal squamous cell carcinoma (LSCC).
Methods: Expression of ATF-3 was examined using immunohistochemistry methods in samples from 83 cases of LSCC carcinoma. MTT
assay was used to detect proliferation of Hep-2 cells after ATF-3 knocked down by siRNA lentivirus. A mouse model was used to investigate
the inhibitive role of ATF-3 siRNA in LSCC xenografts. Realtime RCR was used to detect Cyclin D1 expression after ATF-3 downregulation in
Hep-2 cells. Results: The expression of ATF-3 was positively detected in all the 83 cases of LSCC cancer tissues while Only 4 cases of
adjacent non-neoplastic tissues were detected with positive ATF-3 expression. The ATF-3 expression was statistically related with T stage,
neck nodal metastasis, clinical stage and prognosis of LSCC. Both cell proliferation in vitro and tumor growth in vivo were suppressed after
ATF-3 knockdown. Furthermore, the expression of Cyclin D1 was decreased after ATF-3 downregulation in Hep-2 cells. Conclusion: ATF-3 is
involved in the progress of LSCC, and may provide clinical information for evaluation of prognosis of LSCC. The oncologic role of ATF-3 may be
correlated with Cyclin D1 regulation. (IJCEP1307051).

Keywords: Laryngeal squamous cell carcinoma, activating transcription factor 3, Cyclin D1

Address correspondence to: Dr. Yanan Sun and Dr. Ming Liu, Department of Otorhinolaryngology, Head and Neck Surgery, The Second
Affiliated Hospital, Harbin Medical University, Harbin 150081, PR China. E-mail: syn2767@126.com