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Int J Clin Exp Pathol 2013;6(10):2056-2063
Decreased SARI expression predicts poor prognosis of Chinese patients with non-small
cell lung cancer
Ren-Jie Zhou, Zhong Shi, Kun Zhou, Hai-Dong Wang, Guo-Qiang Zhang, Xue-Tao Li, Jian-Ping Xu
Department of Emergency, Xinqiao Hospital, Third Military Medical University, No. 183, Xinqiaozheng Rd, Chongqing 400037, China;
Department of Thoracic Surgery, Southwest Hospital, Third Military Medical University, No.30, Gaotanyanzheng Rd, Chongqing 400038, China;
Department of Thoracic Surgery, Xinqiao Hospital, Third Military Medical University, No. 183, Xinqiaozheng Rd, Chongqing 400037, China;
Department of Surgery, Chongqing Medical and Pharmaceutical College, No. 82, University zhong Rd, Shapingba district, Chongqing 401331,
China; Department of Pathologists, Xinqiao Hospital, Third Military Medical University, No. 183, Xinqiaozheng Rd, Chongqing 400037, China.
Received August 1, 2013; Accepted August 23, 2013; Epub September 15, 2013; Published October 1, 2013
Abstract: SARI is associated with the risk for several cancers, and loss of SARI expression is frequently found in aggressive and metastatic
cancer. Limited evidence shows that SARI is a tumor suppressor gene, but the role of SARI in non-small cell lung cancer (NSCLC) has not
been previously reported. This study was to investigate the SARI expression profile in surgically resected lung cancer tissues of Chinese
patients by immunohistochemistry and evaluate the relationship between SARI expression and prognosis of lung cancer patients.
Furthermore, SARI gene was transfected into lung cancer cells (A549), and the growth curve and cell healing of lung cancer cells were
determined, aiming to investigate the influence of SARI on the growth and migration of lung cancer cells in vitro. Results showed that 103 of
195 (52.82%) tissues were positive for SARI. When compared with normal tissues, SARI expression significantly reduced in 50.26% of NSCLC
tissues. Patients with negative or reduced SARI expression were more likely to have advanced lung cancer and lymph node metastasis. In
squamous carcinoma and adenocarcinoma patients, the SARI expression had no relation with the survival time; However in one-on-one
analysis SARI expression in tumor cells and adjacent tissues, patients which tumor cells SARI express reduced than adjacent tissues, survival
time was significantly shorter than those without reduction in SARI expression (Log Rank test, p = 0.001). After transfection by SARI gene, the
proliferation and migration of A549 cells were obviously inhibited (p < 0.001). These results demonstrate that decreased SARI expression may
predict a poor prognosis in NSCLC patients, and SARI may serve as a prognostic biomarker and potential therapeutic target for lung cancer.
Keywords: SARI, non-small cell lung cancer, prognosis, surgically resected cancer
Address correspondence to: Ren-Jie Zhou, Department of Emergency, Xinqiao Hospital, Third Military Medical University, No. 183,
Xinqiaozheng Rd, Chongqing 400037, China. Tel: +86-23-68755653; Fax: +86-23-68755653; E-mail: email@example.com