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Int J Clin Exp Pathol 2013;6(10):2092-2101

Original Article
Differential expression and subcellular localization of Prohibitin 1 are related to
tumorigenesis and progression of non-small cell lung cancer

Ping Jiang, Yang Xiang, Yan-Jie Wang, Si-Man Li, Yan Wang, Hai-Rong Hua, Guo-Yu Yu, Yong Zhang, Wen-Hui Lee, Yun Zhang

Key Laboratory of Animal Models and Human Disease Mechanisms of The Chinese Academy of Sciences & Yunnan Province, Kunming
Institute of Zoology, Chinese Academy of Sciences, Kunming, Yunnan 650223, China; University of Chinese Academy of Sciences, Beijing
100049, China; Department of Pathology and Pathophysiology, Kunming Medical University, Kunming, Yunnan 650500, China; Department of
Biochemistry, Kunming Medical University, Kunming, Yunnan 650500, China; Department of Pathology, The Secondly Affiliated Hospital of
Kunming Medical University, Kunming 650032, China

Received August 3, 2013; Accepted August 18, 2013; Epub September 15, 2013; Published October 1, 2013

Abstract: Lung cancer remains the leading cause of cancer-related deaths worldwide and non-small cell lung cancer (NSCLC) accounts for
approximately 85% of all lung cancer. With a variety of biological functions, Prohibitin1 (PHB1) has been proved tumor-associated. But there are
conflicting data regarding the involvement of PHB1 in tumorigenesis and few studies regarding the role of PHB1 in lung cancer. The studies
reported herein used a combination of clinical observations and molecular methods to investigate the possible role of PHB1 in NSCLC tissues
and cell lines. PHB1 expression was evaluated by RT-PCR, RT-qPCR, Western blotting and immunohistochemistry analysis. Flow cytometric
analysis was used to determine the surface expression profiles of PHB1 in lung cell lines. The results showed that PHB1 expression were
generally increased in lung cancer tissues when compared with matched noncancerous tissues and closely related with tumor differentiation
and lymph node invasion. PHB1 expression levels was also increased in three lung cancer cell lines (SK-MES-1, NCI-H157 and NCI-H292) as
compared with BEAS-2B cells. Moreover, there were various subcellular localization of PHB1 in different lung cancer cells and the presence of
PHB1 on the surface of lung cancer cells was significantly reduced. In conclusion, PHB1 expression is increased in NSCLC and the
up-regulation of PHB1 is associated with clinically aggressive phenotype. The different subcellular localization of PHB1 in NSCLC cells and the
loss of the membrane-associated PHB1 probably related to the tumorigenesis and progression of NSCLC and suggests that PHB1 may play
different roles in various types of NSCLC. (IJCEP1308007).

Keywords: Prohibitin 1, up-regulation, subcellular localization, non-small cell lung cancer

Address correspondence to: Dr. Yun Zhang, Key Laboratory of Animal Models and Human Disease Mechanisms of The Chinese Academy of
Sciences & Yunnan Province, Kunming Institute of Zoology, Chinese Academy of Sciences, 32 East Jiao Chang Road, Kunming, Yunnan
650223, China. Tel: +86-871-65198515; Fax: +86-871-65198515; E-mail: zhangy@mail.kiz.ac.cn