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Int J Clin Exp Pathol 2013;6(10):2112-2120

Original Article
Clinicopathological significance of PTEN and PI3K/AKT signal transduction pathway in
non-small cell lung cancer

Fen Yun, Yongfeng Jia, Xiuxia Li, Li Yuan, Qinnuan Sun, Huiling Yu, Lin Shi, Hongwei Yuan

Department of Pathology, The First Affiliated Hospital of Inner Mongolia Medical University, Huhhot, 010059, China; Shandong Heze Medical
College, Heze, Shandong 274000

Received August 8, 2013; Accepted September 3, 2013; Epub September 15, 2013; Published October 1, 2013

Abstract: A high frequency of mutations at the PTEN locus has been noticed in carcinoma of lung. However, the role of PTEN alternations and
its association with outcome variables in the genesis of lung carcinoma are not understood fully. The purpose of our study was to examine the
impact of EGFR, TGF-α, P-AKT and PTEN in the genesis of non-small cell lung cancer (NSCLC). Total numbers of 66 histopathologically
confirmed cases of NSCLC and 10 cases of benign control samples embedded with wax were studied. We assessed EGFR, TGF-α and P-
AKT by the use of specific antibody through immunohistochemistry as directed by the manufacturer, and detected PTEN expression by in situ
hybridization. There were progressive loss of PTEN expression and significant increasing in EGFR, TGF-α, P-AKT expression from benign
samples to NSCLC (p<0.05). The overexpression of EGFR, TGF-α, P-AKT and loss of PTEN expression were correlated to differentiation extent
of cancer tissue, metastasis of lymph nodes and histological classification. Thus, alteration of EGFR, TGF-α, P-AKT and PTEN are likely
important molecular events in pathogenesis and carcinogenesis of NSCLC. (IJCEP1308017).

Keywords: Non-small cell lung cancer (NSCLC), P-AKT, TGF-α, EGFR, PTEN

Address correspondence to: Dr. Yongfeng Jia, Department of Pathology, The First Affiliated Hospital of Inner Mongolia Medical University, NO.
1, Northern Tongdao Street, Huhhot, 010059, China. E-mail: yfjia0479@163.com