IJCEP Copyright © 2007-All rights reserved.
Int J Clin Exp Pathol 2013;6(11):2404-2411

Original Article
DGKα DNA vaccine relieves airway allergic inflammation in asthma model possibly via
induction of T cell anergy

Yan Wang, Qiao Zhang, Qianli Ma, Youlan Zhang, Zhiwei Li, Changzheng Wang

Institute of Respiratory Diseases, Xinqiao Hospital, Third Military Medical University, Chongqing, 400037, China

Received August 12, 2013; Accepted October 8, 2013; Epub October 15, 2013; Published November 1, 2013

Abstract: Induction of T cell immune tolerance is thought to be a good method for treatment of asthma. Diacylglycerol kinases alpha (DGKα),
enzymes that catalyze phosphorylation of diacylglycerol to produce phosphatidic acid, could inhibit diacylglycerol (DAG)-mediated signaling
following T-cell receptor engagement and prevent T cell hyperactivation, thus playing important roles in the induction of T cell anergy. In the
present study, we aimed to investigate the effects of DNA vaccine encoding DGKα gene administration on allergen-induced airway allergic
inflammation in the murine model of asthma. Animal models were created and plasmid containing DGKα were constructed. Cytokine
production was detected after the administration of DGKα gene plasmid. Immunization of mice with alum-adsorbed ovalbumin (OVA) followed
by challenged with inhalation of aerosolized OVA resulted in the development of airway allergic inflammation. Administration of DGKα gene
before the aerosolized OVA challenge significantly decreased the allergic airway inflammation and eosinophil infiltration in bronchoalveolar
lavage fluid (BALF). Immunization with DGKα DNA vaccine decreased OVA-specific IgE and interleukin 13 (IL-13) levels in sera, and increased
the IFN-γ level in BALF. The results of the present study provide evidence for the potential utility of the administration of DGKα DNA vaccine as
an approach to gene therapy for asthma. (IJCEP1308025).

Keywords: Asthma, diacylglycerol kinases alpha, airway inflammation, DNA vaccine

Address correspondence to: Dr. Changzheng Wang or Dr. Yan Wang, Institute of Respiratory Diseases, Xinqiao Hospital, Third Military
Medical University, Chongqing, 400037, China. E-mail: czwang@netease.com (Changzheng Wang); wangyansyd@yahoo.com (Yan Wang)