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Int J Clin Exp Pathol 2013;6(11):2412-2418

Original Article
In vivo biocompatibility of the PLGA microparticles in parotid gland

Mario Cantín, Patricio Miranda, Iván Suazo Galdames, Daniela Zavando, Patricia Arenas, Luis Velásquez, Cristian Vilos

CIMA, Department of Integral Dentistry, Faculty of Dentistry, Doctoral Program in Morphological Science, Universidad de La Frontera, Temuco,
Chile; Center of Research in Biomedical Sciences, Universidad Autónoma de Chile, Temuco, Chile; Private Practice, Chile; Center for the
Development of Nanoscience and Nanothechnology (CEDENNA), Santiago, Chile; Universidad Andres Bello, Facultad de Medicina, Center for
Integrative Medicine and Innovative Science, Santiago, Chile

Received August 13, 2013; Accepted September 3, 2013; Epub October 15, 2013; Published November 1, 2013

Abstract: Poly(lactic-co-glycolic acid) (PLGA) microparticles are used in various disorders for the controlled or sustained release of drugs, with
the management of salivary gland pathologies possible using this technology. There is no record of the response to such microparticles in the
glandular parenchyma. The purpose of this study was to assess the morphological changes in the parotid gland when injected with a single
dose of PLGA microparticles. We used 12 adult female Sprague Dawley rats (Rattus norvegicus) that were injected into their right parotid gland
with sterile vehicle solution (G1, n=4), 0.5 mg PLGA microparticles (G2, n=4), and 0.75 mg PLGA microparticles (G3, n=4); the microparticles
were dissolved in a sterile vehicle solution. The intercalar and striated ducts lumen, the thickness of the acini and the histology aspect in terms
of the parenchyma organization, cell morphology of acini and duct system, the presence of polymeric residues, and inflammatory response
were determined at 14 days post-injection. The administration of the compound in a single dose modified some of the morphometric
parameters of parenchyma (intercalar duct lumen and thickness of the glandular acini) but did not induce tissue inflammatory response,
despite the visible presence of polymer waste. This suggests that PLGA microparticles are biocompatible with the parotid tissue, making it
possible to use intraglandular controlled drug administration. (IJCEP1308028).

Keywords: Salivary glands, parotid gland, drug delivery, microparticles, PLGA

Address correspondence to: Dr. Mario Cantín, CIMA, Department of Integral Dentistry, Faculty of Dentistry, Doctoral Program in Morphological
Science, Universidad de La Frontera, Avenue Manuel Montt 112, Temuco, Chile. Tel: 056-045-2325574; E-mail: mario.cantin@ufrontera.cl