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Int J Clin Exp Pathol 2013;6(11):2292-2299

Original Article
New bone formation in a bone defect associated to dental implant using absorbable or
non-absorbable membrane in a dog model

Maria de Almeida Lopez, Sergio Olate, Antonio Lanata-Flores, Leandro Pozzer, Lucas Cavalieri-Pereira, Mario Cantín, Bélgica Vásquez, José
de Albergaria-Barbosa

Division of Oral and Maxillofacial Surgery, Piracicaba Dental School, State University of Campinas, Brazil; Division of Oral and Maxillofacial
Surgery, Dental School, Universidad de La Frontera, Chile; Center for Biomedical Research, Universidad Autónoma de Chile, Chile; Center for
Research, Universidad de Tarapaca, Chile

Received August 16, 2013; Accepted September 12, 2013; Epub October 15, 2013; Published November 1, 2013

Abstract: The aim of this research was to determine the bone formation capacity in fenestration defects associated with dental implants using
absorbable and non-absorbable membranes. Six dogs were used in the study. In both tibias of each animal 3 implants were installed, and
around these 5 mm circular defects were created. The defects were covered with absorbable membranes (experimental group 1), non-
absorbable membranes (experimental group 2), and the third defect was not covered (control group). At 3 and 8 weeks post-surgery, the
animals were euthanized and the membranes with the bone tissue around the implants were processed for histological analysis. The
statistical analysis was conducted with Tukey’s test, considering statistical significance when p<0.1. Adequate bone repair was observed in the
membrane-covered defects. At 3 weeks, organization of the tissue, bone formation from the periphery of the defect and the absence of
inflammatory infiltrate were observed in both experimental groups, but the defect covered with absorbable membrane presented statistically
greater bone formation. At 8 weeks, both membrane-covered defects showed adequate bone formation without significant differences,
although they did in fact present differences with the control defect in both periods (p>0.1). In the defects without membrane, continuous
connective tissue invasions and bone repair deficiency were observed. There were no significant differences in the characteristics and volume
of the neoformed bone in the defects around the implants covered by the different membranes, whereas the control defects produced
significantly less bone. The use of biological membranes contributes to bone formation in three-wall defects. (IJCEP1308046).

Keywords: Bone regeneration, biological membrane, bone defect

Address correspondence to: Dr. Sergio Olate, Facultad de Odontología, Universidad de La Frontera, Claro Solar 115, 4to Piso, Oficina 20,
Temuco, Chile. E-mail: sergio.olate@ufrontera.cl