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Int J Clin Exp Pathol 2013;6(11):2554-2559
Correlation between chemosensitivity to anticancer drugs and Bcl-2 expression in
Ming Geng, Lin Wang, Peifeng Li
Department of Pathology, General Hospital of Jinan Military Command, Jinan, China; Department of Laboratory Diagnosis, General Hospital of
Jinan Military Command, Jinan, China. Equal contributors.
Received August 23, 2013; Accepted September 20, 2013; Epub October 15, 2013; Published November 1, 2013
Abstract: Objective: To investigate chemoresistance of human gastric cancer to chemotherapeutic drugs in vitro and explore the relationship
with Bcl-2 protein expression. Methods: Single-cell suspensions were prepared from freshly excised samples of primary gastric cancer, and
were separately exposed to taxol (TAX), cisplatin (CDDP), 5-fluorouracil (5-FU), adriamycin (ADM) and mitomycin (MMC) for 48 h. The induction
of cell death was confirmed by microscopic analysis of cell morphology. Metabolic activity and the inhibitory rate (IR) of cells were evaluated by
MTT assay. Expression of Bcl-2 was determined by immunohistochemistry of gastric cancer tissue samples. Results: The IRs of cancer cells
exposed to different chemotherapeutic drugs varied as follows: the IRs for TAX, CDDP and 5-FU were significantly higher than those for ADM
and MMC (P < 0.01). Poorly differentiated gastric cancer cells were more sensitive than well-differentiated cells (P = 0.021). The positive rate of
Bcl-2 expression was 80%, and Bcl-2 expression was significantly associated with chemoresistance to 5-FU (rs = 0.265, P = 0.041), ADM (rs =
0.425, P = 0.001) and MMC (rs = 0.40, P = 0.002). Furthermore, Bcl-2 expression was strongly associated with lymph node metastasis in
gastric cancer (P = 0.009). Conclusion: Overexpression of Bcl-2 may predict a loss of the efficacy of the chemotherapy drugs 5-FU, ADM and
MMC in patients with gastric cancer. (IJCEP1308060).
Keywords: Gastric cancer, Bcl-2, chemosensitivity, chemotherapeutic drugs, inhibitory rate
Address correspondence to: Peifeng Li, Department of Pathology, General Hospital of Jinan Military Command, Jinan 250031, China. E-mail: