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Int J Clin Exp Pathol 2013;6(11):2396-2403

Original Article
Expression analysis of URI/RMP gene in endometrioid adenocarcinoma by tissue
microarray immunohistochemistry

Junxia Gu, Yuting Liang, Longwei Qiao, Xiaoyun Li, Xingang Li, Yaojuan Lu, Qiping Zheng

Department of Hematology and Hematological Laboratory Science, School of Medical Science and Laboratory Medicine, Jiangsu University,
Zhenjiang 212013, China; Department of Hematology, Anyang District Hospital, Anyang 455000, China; Department of Anatomy and Cell
Biology, Rush University Medical Center, Chicago, IL 60612, USA

Received August 30, 2013; Accepted September 25, 2013; Epub October 15, 2013; Published November 1, 2013

Abstract: Multiple studies have recently demonstrated the oncogenic property of URI (or RMP, a member of the prefoldin family of molecular
chaperones) during progression of hepatocellular carcinoma, ovarian cancer, and possibly prostate cancer. Most recently, we have shown that
URI/RMP is up-regulated in cervical cancer, another reproductive system tumor beside ovarian and prostate cancers. To investigate if URI/RMP
also plays a role in other reproductive system tumors, especially in endometrioid adenocarcinoma, we analyzed URI/RMP expression in a TMA
(tissue microarray) containing tissues from 30 cases of endometrioid adenocarcinoma (which covers tumor tissues from Grade I through
Grade III) and adjacent endometrium by immunohistochemistry (IHC) and densitometry analysis using image-pro plus 6.0 software. Our
results showed that the mean density of URI/RMP expression in cancerous tissue is slightly higher than that of the adjacent endometrial
tissue, though not statistically significant (p>0.05). There is no significant difference either between the mean density of Grade III cancerous
tissue and that of Grade I and II cancers. Notably, we detected significantly higher signal intensity in cancerous tissue of all 7 Grade III cases
than that of their adjacent endometrial tissue (p<0.05), suggesting a correlation of URI/RMP expression with the differentiation and pathological
classification of endometrioid adenocarcinoma. Together, our results demonstrate the heterogeneous expression of URI/RMP in endometrioid
adenocarcinoma. The higher level of URI/RMP expression in high-grade endometrioid adenocarcinomas compared to tissues of adjacent
endometrium or gland suggests a diagnostic and possibly, a prognostic value of URI/RMP in endometrioid adenocarcinoma. (IJCEP1308073).

Keywords: URI/RMP, tissue microarray, immunohistochemistry, endometrioid adenocarcinoma

Address correspondence to: Dr. Junxia Gu or Dr. Yaojuan Lu, Department of Hematology and Hematological Laboratory Science, School of
Medical Science and Laboratory Medicine, Jiangsu University, Zhenjiang 212013, China. E-mail: junxiagu@aliyun.com (Junxia Gu);
luyaojuan19@gmail.com (Yaojuan Lu)