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Int J Clin Exp Pathol 2013;6(11):2300-2311

Original Article
Expression and clinical significance of the nin one binding protein and p38 MAPK in
prostate carcinoma

Jian-Ping Che, Wei Li , Yang Yan, Min Liu, Guang-Chun Wang, Qian-Yu Li, Bin Yang, Xu-Dong Yao, Jun-Hua Zheng

Department of Urology, Shanghai Tenth People’s Hospital, Tongji University, Shanghai, 200072, China; Department of Histopathology,
Shanghai Tenth People’s Hospital, Tongji University, Shanghai, China; Institute for Regenerative Medicine, Wake Forest University, Winston-
Salem, NC, USA. Equal contributors.

Received September 2, 2013; Accepted September 17, 2013; Epub October 15, 2013; Published November 1, 2013

Abstract: Background: Prostate carcinoma is a major cause of morbidity and mortality. The MAPK Signaling Pathway plays an important role in
multiple tumors, including prostate carcinoma. MAPK signaling is mediated by ERK1/2, JNK and p38 MAPK, which are important in the control
of cell proliferation, differentiation and apoptosis. However, relatively little is known about the regulatory mechanism of p38 MAPK in prostate
cancers. NOB1 is among the most novel topic in MAPK studies currently. Recent studies found its vital role in tumor metastasis in glioblastoma
proliferation, however, its expression profile and its prognostic value in prostate carcinoma have not been investigated. Methods: To determine
the relationship between NOB1 and p38 MAPK expressions, a population-based study was conducted for immunohistochemical staining
analysis of tumor tissues, in matched malignant and nonmalignant prostatectomy samples from 132 PCa patients. Moreover, Western blot
analysis and NOB1 interference studies of prostate cancer cell lines. To evaluate the diagnostic and prognostic between NOB1 and p38 MAPK
in prostate cancer (PCa) tissue after radical prostatectomy, the hypothesis that prostate cancers with NOB1 expression have distinct clinical,
prognostic and molecular attributes was tested. Results: Among 132 prostate cancers, NOB1 expression was detected in 117 (88.7%) tumors
by immunohistochemistry. NOB1 and p38 MAPK expression had significant positive correlation with carcinogenesis, tumor progression and
patient survival. Immunohistochemically, NOB1 expression in prostate cancer was independently associated with p38 MAPK activation (P=0.
0002). Furthermore, p38 MAPK expression was completely suppressed by NOB1 interference in the prostate cancer cell lines DU-145 and PC-
3. Conclusions: NOB1 expression status was closely correlated with important histopathologic characteristics and the recurrence and
metastasis of prostate carcinomas. These data support a potential link between NOB1 and p38 MAPK, and suggest that NOB1 may identify a
subset of prostate cancer patients with a poor prognosis. This study proved that NOB1 in PCa tissue can be used, in combination with
traditional clinicopathological factors, as promising diagnostic and prognostic tools. (IJCEP1309010).

Keywords: Prostate carcinoma, NOB1, p38 MAPK, histopathologic grade, prognostic markers

Address correspondence to: Dr. Jun-Hua Zheng, Department of Urology, Shanghai Tenth People’s Hospital, Tongji University, No 301,
Yanchang Mid-Road, Shanghai 200072, China. E-mail: liweitongji@163.com