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Int J Clin Exp Pathol 2013;6(11):2451-2459

Original Article
Proteomic analysis of knock-down HLA-G in invasion of human trophoblast cell line

Haiyan Liu, Xueyuan Liu, Hong Jin, Fengying Yang, Weirong Gu, Xiaotian Li

Obstetrics and Gynecology Hospital, Fudan University, Shanghai 200012, China; Department of Chemistry, Fudan University, Shanghai
200433, China; Institute of Biomedical Science, Fudan University, Shanghai 200011, China

Received September 3, 2013; Accepted October 5, 2013; Epub October 15, 2013; Published November 1, 2013

Abstract: Previous studies showed that aberrant HLA-G expression in trophoblast cells plays important roles in trophoblast invasion; however,
the mechanisms remain to be explored. In this study, we found that suppressed HLA-G expression could dramatically decrease the mRNA and
protein expression levels of matrix metalloproteinase 2 and matrix metalloproteinase 9, and in the proteome assay, there were 3 identified
proteins namely, prefoldin 1, eukaryotic translation elongation factor 2 and malate dehydrogenase 2, which were verified by Western blot and
known to be associated with invasion, cell cycle and cell metabolism, respectively. Collectively, our study indicated a potential involvement of
HLA-G in autocrine networks that may regulate prefoldin, MMPs and trophoblast invasion at the maternal-fetal interface in human pregnancy.

Keywords: HLA-G, invasion, proteomics, trophoblast, MMPs

Address correspondence to: Dr. Weirong Gu, Obstetrics and Gynecology Hospital, Fudan University, 419 Fangxie Road, Shanghai 200012,
China. Tel: 86-21-63455050; Fax: 86-21-63457331; E-mail: guweirongdoctor@163.com