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Int J Clin Exp Pathol 2013;6(11):2506-2514

Original Article
Systemic mesenchymal stem cells reduce growth rate of cisplatin-resistant ovarian
cancer

Pengfei Zhu, Mo Chen, Li Wang, Yanxia Ning, Jie Liang, Hao Zhang, Congjian Xu, Sifeng Chen, Liangqing Yao

Department of Gynecology, Obstetrics & Gynecology Hospital, Fudan University, 419 Fangxie Rd, Shanghai, 200011, PR China; Department of
Obstetrics and Gynecology, Shangyu City Hospital, 517 Shimin Blvd Baiguan St, Shangyu, Zhejiang Province, 312000, PR China; Department of
Physiology and Pathophysiology, Shanghai Medical College, Fudan University, 138 Yixueyuan Road, Shanghai, 200032, PR China; Department
of Obstetrics and Gynecology, 2nd Kashgar Regional Hospital, 1 Jiankan Road, Xinjiang Uygur Autonomous Region, 844000, PR China. Equal
contributors.

Received September 7, 2013; Accepted October 12, 2013; Epub October 15, 2013; Published November 1, 2013

Abstract: Epithelial ovarian cancer is one of the most malignant cancers in women and resistant to chemotherapy is the major obstacle for the
five-year survival rate. Cisplatin is one of the effective anticancer drug used in the ovarian cancer. To find a good strategy to cure the tumors
which is resistant to cisplatin, the cisplatin-resistant 3SKOV3 cells were selected from SKOV-3 ovarian cancer cells. Furthermore, the isolated
mesenchymal stem cells were infused systemically to try to cure the transplanted tumor induced by 3SKOV3 cells in nude mice. The
morphology and cell membrane CD44 expression were investigated by microscope and flow cytometry. The biological behaviors of resistant
3SKOV3 and its parental SKOV3 cells, including proliferation, adhesion, and cell cycle were determined by CCK8, absorbance assay and FCM
methods. The transplanted tumors were set up in nude mice with 3SKOV3 cells injection. The growth rate of transplanted tumors was detected
following with MSCs injection. The 3SKOV3 cells have different morphologic manifestation and expressed high level of CD44 molecule. At the
same time, 3SKOV3 cells have less adhesion ability and less S-phase ratio. The isolated MSCs from bone marrow could inhibit the growth of
transplanted tumor via systemic injection. The cisplatin-resistant 3SKOV3 cells have the different biological behaviors as its parental SKOV3
cells. The present study indicated that systemic MSCs have the therapeutic role on ovarian cancer. However, further investigations are in
progress to elucidate the underlying mechanism. (IJCEP1309022).

Keywords: Cisplatin, resistance, ovarian cancer, mesenchymal stem cells, nude mice

Address correspondence to: Dr. Liangqing Yao, Department of Gynecology, Obstetrics & Gynecology Hospital, Fudan University, 419 Fangxie
Rd, Shanghai, 200011, China. Tel: +86-13916644444; E-mail: yaoliangqing@163.com; Dr. Sifeng Chen, Department of Physiology and
Pathophysiology, Shanghai Medical College, Fudan University, 138 Yixueyuan Road, Shanghai, 200032, PR China. Tel: +86-15900577521;
E-mail: chen1216@fudan.edu.cn