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Int J Clin Exp Pathol 2013;6(11):2386-2395

Original Article
Hemocoagulase atrox reduces vascular modeling in rabbit carotid artery adventitia

Sheng-Yun Wan, Yuan-Cheng Hu, Yan-Qing Zhan, Dan-Dan Qin, Yang Ding

Department of Vascular Surgery, The Second Affiliated Hospital of Anhui Medical University, Hefei, Anhui 230601, PR China. Equal contributors.

Received September 8, 2013; Accepted October 10, 2013; Epub October 15, 2013; Published November 1, 2013

Abstract: Objective: This study aimed to compare the effects of hemocoagulase atrox and cauterization hemostasis on intimal hyperplasia and
explore the effect of hemocoagulase atrox on vascular modeling in rabbit carotid artery adventitia. Methods: A total of 27 rabbits were randomly
divided into 3 groups (0d, 14d, 28d). They were anaesthetized using an intramuscular injection of phenobarbital sodium (1 ml/kg). The left and
right common carotid arteries were exposed and capillary hemorrhaged after blunt dissection of the adventitia layers of common carotid
arteries. Nine rabbits in each group were again randomly divided into 3 groups, in which animals were respectively treated with
hemocoagulase (2 U/ml), cauterization (power = 40 w) and saline (as control). Groups of animals were euthanized at 0, 14 and 28 days after
surgery. The samples were equally divided in the middle of the adventitia removal section to obtain equal parts for histologic,
immunohistochemical and molecular biologic analysis. The vascular repair after adventitial stripping was observed by HE staining, Masson
staining and transmission electron microscopy. The expression of carotid MCP-1, PCNA, TGF-β1, α-SMA and VEGF were measured at different
time points by RT-PCR and immunohistochemical staining. Results: HE staining and Masson staining showed that hemocoagulase atrox had
a significantly stronger effect on reducing intimal hyperplasia than the cauterization after 14 and 28 days. The results of RT-PCR showed that
the expression of MCP-1, TGF-β1, α-SMA and VEGF in hemocoagulase atrox-treated animals were lower than that of cauterization-treated
animals. Conclusion: Our results suggested that hemocoagulase atrox as a topical hemostatic is safety and efficiently and it can accelerate
adventitia restoration and decrease intimal proliferation. (IJCEP1309023).

Keywords: Adventitia, hemocoagulase, hemostasis, cauterization, vascular remodeling

Address correspondence to: Dr. Yuan-Cheng Hu, Department of Vascular Surgery, The Second Affiliated Hospital of Anhui Medical University,
Hefei, Anhui 230601, China. Tel: +86-551-63869520; Fax: +86-551-63869520; E-mail: yuanchenghu@163.com