IJCEP Copyright © 2007-All rights reserved.
Int J Clin Exp Pathol 2013;6(12):2896-2903

Original Article
Bee venom ameliorates compound 48/80-induced atopic dermatitis-related symptoms

Kyung-Hyun Kim, Woo-Ram Lee, Hyun-Jin An, Jung-Yeon Kim, Hyun Chung, Sang-Mi Han, Myeong-Lyoel Lee, Kwang-Gill Lee, Sok Cheon Pak,
Kwan-Kyu Park

Department of Pathology, Catholic University of Daegu, College of Medicine, Daegu, 705-718, Republic of Korea; Department of Dermatology,
Catholic University of Daegu, College of Medicine, Daegu, 705-718, Republic of Korea; Department of Agricultural Biology, National Academy of
Agricultural Science, Suwon, 441-100, Republic of Korea; School of Biomedical Sciences, Charles Sturt University, Bathurst, NSW 2795,
Australia

Received September 24, 2013; Accepted October 21, 2013; Epub November 15, 2013; Published December 1, 2013

Abstract: Honeybee (Apis mellifera L.) venom (BV) has been traditionally used for the treatment of pain and inflammatory diseases such as
itchy skin problems. However, the precise mechanism of BV in ameliorating the scratching behavior is not fully understood. Objective: In order
to evaluate the effect of BV on atopic dermatitis-related symptoms in mice, we used a mouse skin scratching model induced by compound
48/80. The anti-itch effect of BV was investigated in a compound 48/80-induced mouse scratching behavior model. BALB/c mice were injected
intraperitoneally with vehicle (saline 0.9%) or BV (0.01 and 0.1 mg/kg). One hour after treatment, the animals received a subcutaneous injection
of compound 48/80. Intraperitoneal administration of BV (0.01 and 0.1 mg/kg) attenuated compound 48/80-induced scratching behaviors. The
anti-scratching behavior effect of BV was in proportional to its vascular permeability effects. Treatment with BV also inhibited the degranulation
of mast cells and the production of pro-inflammatory cytokines in compound 48/80-treated skin tissues. According to these results, BV may
improve atopic dermatitis-related symptoms by inhibiting the mast cell degranulation and pro-inflammatory cytokine expression.
(IJCEP1309061).

Keywords: Bee venom, scratching behavior, mast cell degranulation, compound 48/80, inflammation

Address correspondence to: Dr. Kwan-Kyu Park, Department of Pathology, Catholic University of Daegu, College of Medicine, 3056-6
Daemyung 4-Dong, Nam-Gu, Daegu, 705-718, Republic of Korea. Tel: +82-53-650-4149; Fax: +82-53-650-4834; E-mail: kkpark@cu.ac.kr