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Int J Clin Exp Pathol 2013;6(12):2824-2834

Original Article
MiR-195 inhibits proliferation and growth and induces apoptosis of endometrial stromal
cells by targeting FKN

Yun Wang, Hong Chen, Yonglun Fu, Ai Ai, Songguo Xue, Qifeng Lyu, Yanping Kuang

Department of Assisted Reproductive, Shanghai Ninth Hospital Affiliated to JiaoTong University School of Medicine, Shanghai, China. Equal
contributors.

Received September 9, 2013; Accepted September 24, 2013; Epub November 15, 2013; Published December 1, 2013

Abstract: MiR-195, which exhibits a proliferation-inhibiting role in different tumors, has been reported to be down-regulated in the ectopic
endometrium. The aim of this study was to determine the impact of miR-195 on the biological characteristic of the endometrial stromal cells
(ESCs). MiR-195 has been presumed to target the 3’-untranslated regions (3’-UTR) of Fractalkine (FKN), which also plays important roles in
endometriosis. Fluorescence reporter assays showed that miR-195 effectively binds to the 3’-UTR of FKN. The normal ESCs showed a
significant higher miR-195 expression than that of eutopic and ectopic ESCs associated with endometriosis, while the FKN expression
showed opposite results. MiR-195 mimics inhibited proliferation and growth and induced apoptosis of eutopic ESCs, and these effects were
abolished by FKN-siRNA. miR-195 could decrease the expression of survivin, matrix metalloproteinase -9 (MMP9) and up-regulate the
expression of CD82, tissue inhibitor of metalloproteinase 1 (TIMP1) and TIMP2 of eutopic ESCs by targeting FKN. Our study has demonstrated
for the first time that miR-195 plays important roles in regulating the functions of ESCs through targeting FKN. The information may be useful
for developing a new therapeutic strategy for endometriosis. (IJCEP1310024).

Keywords: MiR-195, endometriosis, endometrial stromal cells, fractalkine

Address correspondence to: Dr. Qifeng Lyu and Dr. Yanping Kuang, Department of Assisted Reproductive, Shanghai Ninth Hospital Affiliated
to JiaoTong University School of Medicine, No. 639, Zhizhao Ju Road, 200011, Shanghai, China. Tel: 86-21-23271699-5538; Fax: 86-21-
53078108; E-mail: lyuqifeng@126.com (Qifeng Lyu); kuangyanp@126.com (Yanping Kuang)