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Int J Clin Exp Pathol 2013;6(12):2757-2764

Original Article
Development of neural stem cells at different sites of fetus brain of different gestational
age

Xiaojuan Yin, Lihua Li, Xiaoying Zhang, Yao Yang, Yannan Chai, Xiao Han, Zhichun Feng

Affiliated Bayi Children’s Hospital, Beijing Military Region General Hospital, No 5, Nan Mencang, Dongcheng District, Beijing 100700, China.
Equal contributors.

Received October 11, 2013; Accepted November 7, 2013; Epub November 15, 2013; Published December 1, 2013

Abstract: Objective: This study aimed to investigate the development of neural stem cells (NSCs) in fetal brain, which may provide
experimental evidence for the clinical treatment of brain injury in children. Methods: A total of 60 fetuses were collected after labor induction and
divided into 6 groups according to the gestational age (16 w, 20 w, 24 w, 28 w, 32 w and 36 w; n=10 per group). The hippocampus, striatum,
subventricular zone, frontal lobe, temporal lobe, occipital lobe and parietal lobe were harvested. In situ hybridization, immunohistochemistry
and light microscopy were done to determine the morphology and quantity of NSCs. Results: NSCs were identified in the brain of fetuses with
different gestational age. NSCs were round, oval, spindle-shaped, starlike, triangular or polygonal. NSC colony was also observed with
symmetrical or asymmetrical division. Single NSC, group-like NSCs and cluster-like NSCs were found in the different sites of fetal brain, and
NCSs interacted with each other via synapses. However, the distribution, morphology, growth and quantity of NSCs were different in the brain of
fetuses with different gestational age. The number of NSCs reduced with the increase in gestational age, but they were always observed.
Conclusion: The morphology of NSCs in fetal brain is variable and they are widely distributed in the hippocampus, subventricular zone, striatum
and cortex. The number of NSCs reduced with the increase of gestational age. (IJCEP1310028).

Keywords: Human, fetal brain, nestin

Address correspondence to: Dr. Zhichun Feng or Xiaojuan Yin, Affiliated Bayi Children’s Hospital, Beijing Military Region General Hospital, No
5, Nan Mencang, Dongcheng District, Beijing 100700, P. R. China. E-mail: drfengzc@fmmu.edu.cn (Zhichun Feng); yinxiaojuan11@yeah.net
(Xiaojuan Yin)